-
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoiseExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP eIF2beta C-term (aa 89-331)
Plasmid#45901DepositorInserteIF2S2 aa89-331 (Eif2s2 Mouse)
UseTagsEGFPExpressionMammalianMutationaa 89-331PromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pScSR beta deltaN Flag
Plasmid#13729DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHis and FlagExpressionBacterialMutationexpresses residues 31-244 of S. cerevisiae SRβ wi…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)
Plasmid#119826PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-G610C
Plasmid#119827PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCVpuro-6xHis_SUMO2
Plasmid#164938PurposeExpresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNADepositorInsertSmall Ubiquitin Like Modifier 2 (SUMO2 Human)
UseRetroviralTags6XHISExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα2(I)-G610C
Plasmid#119839PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)
Plasmid#119840PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)-G610C
Plasmid#119837PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα2(I)
Plasmid#119838PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-G610C
Plasmid#119841PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)
Plasmid#119836PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TGmiR-Gb2
Plasmid#25746PurposeControl lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.DepositorInsertGb2 (Gnb2 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Gb2
Plasmid#25744PurposeControl lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertGb2 (Gnb2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntr_PE2
Plasmid#149548PurposeGateway entry clone with PE2 enzyme (with stop codon)DepositorInsertPE2
UseCRISPR; Gateway shuttle vectorTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Giardia Dicer
Plasmid#89146PurposeExpression of Giardia Dicer in Sf9 cellsDepositorInsertGiardia Dicer
UseTagsHis6-teveExpressionInsectMutationPromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-HTNC
Plasmid#13763PurposeFor expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.DepositorInsertHis-TAT-NLS-Cre
UseBaculovirusTagsHis Tag, NLS, and TATExpressionBacterialMutationPromoterp10 promoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pLV-mScarlet-Control-5'UTR
Plasmid#184637PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits