162,309 results

1. hFerritinH

   Plasmid

   #122652

   Purpose

   Expression of hFerritinH in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PG13-luc (wt p53 binding sites)

   Plasmid

   #16442

   Purpose

   Luciferase reporter containing 13 copies of the p53-binding consensus sequence

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-miRFP670nano3

   Plasmid

   #184664

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6-pAG-RTase

   Plasmid

   #214932

   Purpose

   Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

   Depositor

   Chuan He

   Article

   Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

   Insert

   fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

   Promoter

   T7 promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-TEAD1

    Plasmid

    #229411

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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