159,557 results

1. pAAV.hSyn.iGluSnFr.WPRE.SV40

   Plasmid

   #98929

   Purpose

   AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

   Plasmid

   #113865

   Purpose

   Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-iRFP
   PTCH1-mTurquoise2
   CBF-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG148 FRB-NFAST

   Plasmid

   #130812

   Purpose

   Expresses FRB-NFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FRB-NFAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLBPVXBa-M

   Plasmid

   #229079

   Purpose

   New version of a PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation coat protein start codon (ATG to AGG); d…

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. XRE-pNL1.3[secNLuc]

   Plasmid

   #182295

   Purpose

   Secreted Luciferase reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   Xenobiotic Response Element (CYP1A1 Human)
   

   Use

   Tags

   secNluc

   Expression

   Mammalian

   Mutation

   Promoter

   XRE

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-dCas9-LSD1-Hygro

   Plasmid

   #104406

   Purpose

   transient expression of dCas9-LSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   LSD1 (KDM1A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiV_Cas9_Blast

   Plasmid

   #125592

   Purpose

   Lentiviral expression of spCas9 with blasticidin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

   Plasmid

   #50460

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTH102

    Plasmid

    #84460

    Purpose

    pJB38-NWMN29-30 + SarA_P1-CFP-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-CFP from pTH2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge