We narrowed to 8,814 results for: CAG

1. Ai32

   Plasmid

   #34880

   Purpose

   Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   Floxed ChR2(H134R)-EYFP
   

   Use

   Mouse Targeting

   Tags

   EYFP

   Expression

   Mutation

   H134R in ChR2

   Promoter

   CAG

   Available since

   May 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJOP-HTT-HR18Q

   Plasmid

   #87228

   Purpose

   HTT HR arms with 18 CAG repeats and piggyBac selection cassette

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a driving selection cassette

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT105

   Plasmid

   #180510

   Purpose

   Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXN2-FLAG-AT2R-CFP

   Plasmid

   #101660

   Purpose

   Expresses AT2R with FLAG tag and CFP in mammalian cells.

   Depositor

   Yusuke Ohba

   Article

   Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

   Insert

   Angiotensin II type 2 receptor (AGTR2 Human)
   

   Use

   Tags

   ECFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CALID

   Plasmid

   #169708

   Purpose

   Cre-activated light-inducible Dre recombinase

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-PEmax SpG

   Plasmid

   #187895

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, SpG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ai27

   Plasmid

   #34630

   Purpose

   Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   Floxed hChR2(H134R)-tdTomato
   

   Use

   Mouse Targeting

   Tags

   tdTomato

   Expression

   Mutation

   His 134 has been mutated to Arg

   Promoter

   CAG

   Available since

   Feb. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBbsr-6080NES(Epac-sensor)

   Plasmid

   #162487

   Purpose

   hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Epac-sensor (RAPGEF3 Synthetic, Human)
   

   Use

   Tags

   YPet and Turquoise2GL

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PE SpG

    Plasmid

    #179081

    Purpose

    All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36881

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-PE VQR

    Plasmid

    #162797

    Purpose

    All-in-one prime editor piggyBac transposon, VQR variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT526

    Plasmid

    #180513

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-super-retro puro RhoGDI2 shRNA

    Plasmid

    #58897

    Purpose

    produces an shRNA against RhoGDI2

    Depositor

    Channing Der

    Article

    Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

    Insert

    RhoGDI2 (ARHGDIB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Lenti CGIP + M-AAT target

    Plasmid

    #86008

    Purpose

    lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    M-AAT target
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V30M

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB.DEST-ITGB1(YYFF)-mRuby2

    Plasmid

    #215449

    Purpose

    Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Transposon-based stable expression

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Mutations in the NPxY sites Y783F, Y795F; Silent …

    Promoter

    CAG

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pYO1031

    Plasmid

    #235706

    Purpose

    Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Inserts

    Beclin 1 (BECN1 Human)
    UVRAG (UVRAG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYO1101

    Plasmid

    #235708

    Purpose

    Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Inserts

    Beclin 1 (BECN1 Human)
    ATG14L (ATG14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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