We narrowed to 3,807 results for: golden gate

1. pUC57-mini v2-HF24_F23

   Plasmid

   #195737

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#23 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pUC57-mini v2-HF24_F21

   Plasmid

   #195735

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#21 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pYPQ131-RZ-Cas12j2

   Plasmid

   #189782

   Purpose

   Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ132-RZ-Cas12j2

   Plasmid

   #189783

   Purpose

   Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ134-RZ-Cas12j2

   Plasmid

   #189785

   Purpose

   Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB61 - pL0_V2 (CDS1)

   Plasmid

   #123183

   Purpose

   Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGC_RT_mScarlet

   Plasmid

   #136967

   Purpose

   Goldengate/Greengate CDS module, containing mScarlet

   Depositor

   Klaus Theres

   Insert

   mScarlet
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGD_RT_SRDX

   Plasmid

   #136976

   Purpose

   Goldengate/Greengate module EAR-repression domain

   Depositor

   Klaus Theres

   Insert

   SRDX
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGGC_RT_Tq2CFP

   Plasmid

   #136969

   Purpose

   Goldengate/Greengate CDS module, containing Tq2CFP.

   Depositor

   Klaus Theres

   Insert

   Tq2CFP
   

   Expression

   Bacterial

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGGD_RT_2NLS

    Plasmid

    #136977

    Purpose

    Goldengate/Greengate module nuclear localization signal

    Depositor

    Klaus Theres

    Insert

    SV40 NLS
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGA_RT_pLexA

    Plasmid

    #136961

    Purpose

    Goldengate/Greengate module for generation of beta-estradiole inducible genes.

    Depositor

    Klaus Theres

    Insert

    pLexA
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGGD_RT_li_Tq2CFP

    Plasmid

    #136971

    Purpose

    Goldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linker

    Depositor

    Klaus Theres

    Insert

    Tq2CFP
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGGB_RT_Tq2CFP_li

    Plasmid

    #136965

    Purpose

    Goldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linker

    Depositor

    Klaus Theres

    Insert

    Tq2CFP
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGB_RT_Venus_li

    Plasmid

    #136966

    Purpose

    Goldengate/Greengate N-tag module, containing Venus fused to a flexible linker

    Depositor

    Klaus Theres

    Insert

    Venus
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGGB_RT_tdTomato_li

    Plasmid

    #136964

    Purpose

    Goldengate/Greengate N-tag module, containing tdTomato fused to a flexible linker

    Depositor

    Klaus Theres

    Insert

    tandem Tomato
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGC_RT_Sa_sgRNA

    Plasmid

    #136968

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGI_RT_Sa_sgRNA

    Plasmid

    #136982

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGD_RT_Sa_sgRNA

    Plasmid

    #136975

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGD_RT_li_Tq2CFP_2NLS

    Plasmid

    #136972

    Purpose

    Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker

    Depositor

    Klaus Theres

    Insert

    Tq2CFP
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGE_RT_Sa_sgRNA

    Plasmid

    #136978

    Purpose

    Goldengate/Greengate module for genome editing, using the Sa guideRNA.

    Depositor

    Klaus Theres

    Insert

    Sa guide RNA
    

    Use

    CRISPR

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge