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p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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S. aureus DHPS

Plasmid

#64079

Purpose

Expression of Staphylococcus aureus dihydropteroate synthase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA5woH

Plasmid

#65019

Purpose

Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_WTnoGirdles

Plasmid

#62653

Purpose

Wild-type OmpA with aromatic girdle residues replaced by leucine

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_noGirdles

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Aromatic girdle residues mutated to Leu (W7, W15,…

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

Plasmid

#52006

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF1mut

Expression

Mammalian

Mutation

mutation removing the start codon for ORF1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
roGFP_iR_pQE30

Plasmid

#48634

Purpose

Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

roGFP1

Tags

HisTag

Expression

Bacterial

Mutation

insertion R147B

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa

Plasmid

#46166

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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Cyprinodon variegatus cryaa

Plasmid

#47664

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pFA5

Plasmid

#46791

Purpose

To express a version of Fun30 without CUE domain in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 without CUE domain (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only
pFA12

Plasmid

#46792

Purpose

To express a version of Fun30 with a mutated CUE domain in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30, CUE domain mutated (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

FP82AA

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y96F

Plasmid

#47094

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F

Plasmid

#47091

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y96F

Plasmid

#47085

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y91F

Plasmid

#47084

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y91F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y32F Y96F

Plasmid

#47083

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y32F Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y32F

Plasmid

#47082

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y32F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pCR2.1-SNORD116-3

Plasmid

#45891

Depositor

Stefan Stamm

Article

Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

Insert

SNORD115 with 80nt from intron sequence but without flanking exons (SNORD116-3 Human)

Use

Unspecified

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only