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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Smad7

Plasmid

#80893

Purpose

mammalian expression of Smad7

Depositor

Aristidis Moustakas

Insert

Smad7 (Smad7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

aa 2-426

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

Plasmid

#90013

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-Dicer

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Gateway Cloning

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Tags

Histag-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

Plasmid

#90012

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/BLM

Plasmid

#111766

Purpose

To express human BLM protein in human cells

Depositor

Ian Hickson

Article

Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

Insert

BLM (BLM Human)

Tags

Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCag SE (Self-excising) FlpO-2A-Cre EV

Plasmid

#130986

Purpose

episomal expression of FlpO and Cre recombinases, self excising

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

FlpO-2A-Cre

Use

Cre/Lox and Unspecified; Episomal expression vect…

Expression

Mammalian

Mutation

protamine intron added to FlpO to prevent bacteri…

Promoter

CMV/Chick β-actin (CAG)

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-sh-mFAK C

Plasmid

#37015

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

sh-mFAK C (Ptk2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C

Plasmid

#116749

Purpose

Lentiviral expression of GUCY2C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(T21N)

Plasmid

#99711

Purpose

Mammalian expression of HA-RagA(T21N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Mutation

T21N

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Expression

Bacterial

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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