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pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

Plasmid

#195505

Purpose

T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)

Use

CRISPR; Donor vector

Tags

mRuby-3xFLAG-NLS-3xSV40-poly(A)

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-LRP4 (cytoplasmic) [N164/6.1R]

Plasmid

#114542

Purpose

Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

Plasmid

#179432

Purpose

Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

HS4-TRE-cit-HS4-mch (DH)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-AURKB-PGK-mCherry

Plasmid

#153316

Purpose

Expresses AURKB and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

AURKB (AURKB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd5

Plasmid

#102867

Purpose

Expresses mouse Fzd5 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 5 (Fzd5 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

A to G substitution at nucleotide 699 of Fzd5 ORF…

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

Plasmid

#195503

Purpose

SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)

Use

CRISPR; Donor vector

Tags

T2A-H2B-mCitrine

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, alpha6 [N229A/32R]

Plasmid

#164155

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, alpha6 (Mouse). Derived from hybridoma N229A/32.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, alpha6 (Mus musculus) recombinant mouse monoclonal antibody (Gabra6 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Mitofusin-2 [N153/5.1R]

Plasmid

#114547

Purpose

Mammalian Expression Plasmid of anti-Mitofusin-2 (Mouse). Derived from hybridoma N153/5.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mitofusin-2 (Mus musculus) recombinant mouse monoclonal antibody (Mfn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha5 [N415/24R]

Plasmid

#149456

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha5 (Human). Derived from hybridoma N415/24.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Alpha5 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA5 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

Plasmid

#159680

Purpose

Mammalian protein expression of KRAS4a-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4a (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Beta1 [N96/55.3R]

Plasmid

#114549

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Beta1 (Mouse). Derived from hybridoma N96/55.3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Beta1 (Mus musculus) recombinant mouse monoclonal antibody (Gabra1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-DLX1 [L43/40R]

Plasmid

#128633

Purpose

Mammalian Expression Plasmid of anti-DLX1 (Human). Derived from hybridoma L43/40.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-DLX1 (Human) recombinant mouse monoclonal antibody (DLX1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ Channel [K89/34R]

Plasmid

#128628

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ Channel (Rat). Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Mitofusin-1 [N111/24R]

Plasmid

#140072

Purpose

Mammalian Expression Plasmid of anti-Mitofusin-1 (Mouse). Derived from hybridoma N111/24.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mitofusin-1 (Mouse) recombinant mouse monoclonal antibody (Mfn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188901

Purpose

dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

SID-dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC403 LacZ

Plasmid

#111184

Purpose

Control inducible vector with LacZ (V5 tagged)

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

lacZ

Use

Lentiviral

Tags

V5

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC401 LacZ

Plasmid

#111183

Purpose

Control inducible vector with LacZ (V5 tagged)

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

lacZ

Use

Lentiviral

Tags

V5

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.1b K+ channel [N16B/8R]

Plasmid

#114511

Purpose

Mammalian Expression Plasmid of anti-Kv3.1b K+ channel (Rat). Derived from hybridoma N16B/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.1b K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R]

Plasmid

#177512

Purpose

Mammalian Expression Plasmid of anti-GFAP (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFAP (Homo sapiens) recombinant mouse monoclonal antibody (GFAP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 27, 2021

Availability

Academic Institutions and Nonprofits only

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