We narrowed to 5,000 results for: mos

1. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426GAL-ccdB-mRuby3

   Plasmid

   #166142

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Tags

   mRuby3

   Expression

   Yeast

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

   Plasmid

   #208977

   Purpose

   Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

   Plasmid

   #208974

   Purpose

   Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

   Plasmid

   #208970

   Purpose

   Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. hPCNA-C148S

   Plasmid

   #190939

   Purpose

   untagged human PCNA with a cysteine to serine mutation

   Depositor

   Brian Kelch

   Article

   Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Expression

   Bacterial

   Mutation

   changed cysteine 148 to serine

   Promoter

   T7

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

   Plasmid

   #194462

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

   Plasmid

   #194460

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. fru-T2A-QF2 HDR plasmid

    Plasmid

    #141099

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2z-lmo2

    Plasmid

    #164657

    Purpose

    for the synthesis of zebrafish lmo2 mRNA

    Depositor

    Didier Stainier

    Article

    Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

    Insert

    lmo2 (lmo2 Zebrafish)
    

    Use

    Mrna synthesis

    Promoter

    SP6

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ETS2_pLENTI-CAG-IRES-GFP

    Plasmid

    #177005

    Purpose

    Mammalian lentiviral expression vector encoding ETS2

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    ETS2 (ETS2 Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CYYR1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176986

    Purpose

    Mammalian expression vector encoding CYYR1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CYYR1 (CYYR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CBR3_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176961

    Purpose

    Mammalian expression vector encoding CBR3 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CBR3 (CBR3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CLDN8_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176963

    Purpose

    Mammalian expression vector encoding CLDN8 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CLDN8 (CLDN8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. FAM207A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176950

    Purpose

    Mammalian expression vector encoding FAM207A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    FAM207A (C21orf70 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-HLA-A2-VN (G112P)

    Plasmid

    #135496

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    G112P

    Promoter

    CMV

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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