Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,311 results for: puro

Showing: 3601 - 3620 of 18311 results

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pcDNA5-puro-eGFP-HEC1-M2

Plasmid

#114035

Purpose

Expression of HEC1 M2 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M2 (NDC80 Human)

Expression

Mammalian

Mutation

M2 (K81E, N87E, K89E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-9A

Plasmid

#114023

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830E

Plasmid

#91996

Purpose

Expresses FLAG-HA-AGO2 (T830E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834E

Plasmid

#91998

Purpose

Expresses FLAG-HA-AGO2 (S834E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824A

Plasmid

#91987

Purpose

Expresses FLAG-HA-AGO2 (S824A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830A

Plasmid

#91988

Purpose

Expresses FLAG-HA-AGO2 (T830A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S831A

Plasmid

#91989

Purpose

Expresses FLAG-HA-AGO2 (S831A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S831A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834A

Plasmid

#91990

Purpose

Expresses FLAG-HA-AGO2 (S834A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828A

Plasmid

#91991

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E

Plasmid

#91994

Purpose

Expresses FLAG-HA-AGO2 (S824E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828E

Plasmid

#91995

Purpose

Expresses FLAG-HA-AGO2 (S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Showing: 3601 - 3620 of 18311 results

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