165,025 results

1. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Tags

   24×Broccoli

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-HSF1

   Plasmid

   #32537

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMevT

   Plasmid

   #17815

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Promoter

   U6, Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Patrick Salmon, Arthur Nienhuis

   Insert

   Ecotropic MLV envelope
   

   Expression

   Mammalian

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   David Root, John Doench, William Hahn

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Centrin2 (Nigg UK185)

   Plasmid

   #41147

   Depositor

   Erich Nigg

   Article

   Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

   Insert

   Centrin-2 (CETN2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Expression

    Bacterial

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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