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GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Publication

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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hFlt1 V767A

Plasmid

#86038

Purpose

human VEGFR1 with V to A mutation at position 767

Depositor

Christie Thomas

Publication

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 V767A (FLT1 Human)

Tags

FLAG, HA (please see depositor comments below), a…

Expression

Mammalian

Mutation

V767A

Promoter

CMV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-for

Plasmid

#81216

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Expression

Bacterial

Promoter

pBAD

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Publication

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Expression

Mammalian

Promoter

hEF1alpha

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Longest 3' UTR

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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592 p3e-EST etv2 3’UTR

Plasmid

#49003

Purpose

Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

only the 3' UTR

Promoter

3' gateway entry vector

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Publication

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Expression

Bacterial

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBlue-hFX-HA

Plasmid

#14987

Depositor

Michael Ristow

Publication

Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pPS1358

Plasmid

#8918

Depositor

Pamela Silver

Publication

Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

Insert

HRP1 (HRP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pGWB402SC

Plasmid

#224552

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Publication

Lawson et al EMBO J. 2025 Jun;44(11):3210-3230. doi:

Type

Empty backbone

Tags

Twin-Strep-HA

Expression

Plant

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB402SN

Plasmid

#224551

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Publication

Lawson et al EMBO J. 2025 Jun;44(11):3210-3230. doi:

Type

Empty backbone

Tags

HA-Twin-Strep

Expression

Plant

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

Plasmid

#122102

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKM50

Plasmid

#123656

Purpose

Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

Depositor

Kevin Maringer

Publication

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Aedes aegypti UbL40 promoter

Use

Luciferase

Expression

Insect

Promoter

UbL40 promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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