We narrowed to 20,804 results for: ATO

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Mutation

Codon Optimized

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Mito-PyronicSF/pCMV-myc-mito

Plasmid

#124813

Purpose

Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1

Plasmid

#205446

Purpose

human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HSV TK-H2-Kb SCT-mCD47-mCD55

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast tBID-flag

Plasmid

#211533

Purpose

Expresses truncated BID upon doxycycline addition to trigger apoptosis

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

tBID-flag (BID Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated (CASP8) form (p15)

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shPOLE1_1

Plasmid

#160810

Purpose

Express Dox repressible shPOLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

Plasmid

#225708

Purpose

Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Has Service

AAV9

Insert

ASAP5-Kv2.1

Use

AAV

Promoter

EF1α

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Myc-SCAP

Plasmid

#134285

Purpose

Expresses Myc-tagged SCAP in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SCAP (Scap, Scap Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pKI_ΩBBMVP16&WUS

Plasmid

#220348

Purpose

Expresses BBM-VP16 and WUS in plant cells

Depositor

Tomoko Igawa

Article

Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

Inserts

BABY BOOM (BBM Mustard Weed)
WUSCHEL (WUS Mustard Weed)

Tags

VP16 transcriptional activation domain

Expression

Plant

Promoter

CaMV 35S and RPS5A

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pPB_Cdc42-TomKat-sensor-CAAX

Plasmid

#172473

Purpose

Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

Depositor

Sean R Collins

Article

Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

Insert

tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX

Use

Piggybac transposon

Tags

polybasic and CAAX motif from Kras, tdKatushka2, …

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Tags

His

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off blast NFS1

Plasmid

#160801

Purpose

Express Dox repressible NFS1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

NFS1 (NFS1 Human)

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-PyronicSF/pCMV-myc-nuc

Plasmid

#124814

Purpose

Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Tags

Nuclear Localization Signal

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

Plasmid

#205443

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46-HLA-G SCT-hCD47-hCD59

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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