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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pX458-AAVS1-sg

Plasmid

#194721

Purpose

px458 with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

Cas9 (AAVS1 Streptococcus pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCJ111

Plasmid

#175052

Purpose

rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pZHY013

Plasmid

#36185

Depositor

Daniel Voytas

Article

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

Heterodimeric FokI nuclease

Use

Entry vector for expression in plants as a t2a li…

Promoter

none

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pFF838

Plasmid

#61457

Purpose

aTc-inducible SCRIBE_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_lacZ(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTAL7a

Plasmid

#48705

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Promoter

copia

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAP973-1

Plasmid

#99496

Purpose

TEV::linker::meGFP:3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::meGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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