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pCX460

Plasmid

#229215

Purpose

CRISPR array plasmid for MAP1B (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 25-48)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Mutation

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCralbp-DsRed

Plasmid

#11158

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Cralbp promoter (Rlbp1 Mouse)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pTALYM3

Plasmid

#47874

Purpose

Destination plasmid with mClover for Golden Gate reaction of TALE assembly

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Type

Empty backbone

Use

TALEN

Tags

mClover

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVB1.3

Plasmid

#37974

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

pJRTCLCrVB1.3

Use

Agrobacterium binary

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMycDam

Plasmid

#59218

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Mutation

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pKF295

Plasmid

#72871

Purpose

expression of improved FLP recombinase

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

FLP recombinase (flp Budding Yeast)

Use

Tags

Expression

Insect

Mutation

P2->S, added NLS

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-LacZ

Plasmid

#18816

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-LacZ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pTC147

Plasmid

#70013

Purpose

T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

35S:ANT1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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BA-Wnt10b

Plasmid

#1831

Depositor

Philip Leder

Article

Lane et al Oncogene 1997 Oct;15(18):2133-44.

Insert

Wnt10b (Wnt10b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only
F3/-2.5CAT

Plasmid

#25425

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

Insert

Myo D Promoter + F3 (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
-2.5CAT

Plasmid

#25424

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pEGB3_alpha2 35:PhyB:VP16 (GB2745)

Plasmid

#160630

Purpose

TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

Depositor

Diego Orzaez

Insert

PhyBVP16

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF

Plasmid

#26262

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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FoRC.PGa

Plasmid

#84584

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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FoRC.PRa

Plasmid

#84585

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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FoRC.PRm

Plasmid

#84587

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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CJ34 (miR-1003 3'ssAG->GA)

Plasmid

#17770

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila p-element vector

Tags

Expression

Mutation

splice site mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pTALEN-STAR-T

Plasmid

#87522

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-A

Plasmid

#87523

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge