We narrowed to 17,391 results for: puro

1. pJ609 Flag-Sumo1

   Plasmid

   #49046

   Purpose

   Mammalian expression of N-terminally tagged Sumo1

   Depositor

   Lianna Swanson

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTK93_Lifeact-mCherry

   Plasmid

   #46357

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   Lifeact
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL2314

   Plasmid

   #137054

   Purpose

   loxP (empty) expression cassette: c-terminal 6xHA

   Depositor

   Jeremy Mottram

   Article

   Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

   Type

   Empty backbone

   Use

   Leishmania floxed gene expression

   Tags

   6xHA

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p663-UBC-TurboID-V5-dsRed_IDG-K

   Plasmid

   #135261

   Purpose

   Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-dsRed
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAID5.1-N

   Plasmid

   #145808

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Use

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRIPZ (M)-HT-Cbx7

   Plasmid

   #82515

   Purpose

   Lentivirus vector. Expresses Halotag-Cbx7 fusion proteins in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 7 (CBX7 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMA2780

   Plasmid

   #25438

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   TRE-Tight
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. LT3GEPIR-NEK2shRNA2

   Plasmid

   #220541

   Purpose

   To inducibly knockdown NEK2 expression

   Depositor

   Sean Lee

   Insert

   NEK2 shRNA2 (NEK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CLYBL hOPM

   Plasmid

   #112499

   Purpose

   Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

   Depositor

   Bruce Conklin

   Insert

   OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid

    #135275

    Purpose

    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-GFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LT3GEPIR-CDK12shRNA

    Plasmid

    #220583

    Purpose

    To inducibly knockdown CDK12 expression

    Depositor

    Sean Lee

    Insert

    CDK12 shRNA (CDK12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid

    #135264

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCXIP-mCherry-TAZ

    Plasmid

    #128351

    Purpose

    Retrovirus vector to express mCherry-TAZ

    Depositor

    Yutaka Hata

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRIPZ (M)-HT-Cbx6

    Plasmid

    #82514

    Purpose

    Lentivirus vector. Expresses Halotag-Cbx6 fusion proteins in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 6 (CBX6 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX307_CdTag-HA_EmptyVector

    Plasmid

    #221467

    Purpose

    Gateway backbone for C-terminal dTag cloning

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGH045_EGFP

    Plasmid

    #85411

    Purpose

    lentiviral expression vector for GFP

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLB2 CAG P2Gm

    Plasmid

    #19752

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Type

    Empty backbone

    Use

    Lentiviral, Mouse Targeting, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LT3GEPIR-HDACshRNA

    Plasmid

    #220573

    Purpose

    To inducibly knockdown HDAC3 expression

    Depositor

    Sean Lee

    Insert

    HDAC3 shRNA (HDAC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LT3GEPIR-NEK1shRNA2

    Plasmid

    #220539

    Purpose

    To inducibly knockdown NEK1 expression

    Depositor

    Sean Lee

    Insert

    NEK1 shRNA2 (NEK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSMPP

    Plasmid

    #104970

    Purpose

    Lentiviral Transfer Plasmid empty backbone

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid

    #135240

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5-miniTurbo
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAID5.2-N

    Plasmid

    #145810

    Purpose

    An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

    Type

    Empty backbone

    Use

    Tags

    mAID-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX302_CdTag-HA_EmptyVector

    Plasmid

    #221465

    Purpose

    Gateway backbone for C-terminal dTag cloning

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p667-UBC-hcRed-V5-miniTurbo_IDG-K

    Plasmid

    #135237

    Purpose

    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    hcRed-V5-miniTurbo
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAVS hOPM

    Plasmid

    #112498

    Purpose

    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.

    Depositor

    Bruce Conklin

    Insert

    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAID5.2-C

    Plasmid

    #145811

    Purpose

    An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

    Type

    Empty backbone

    Use

    Tags

    mAID-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p663-UBC-PM-miniTurbo-V5_IDG-K

    Plasmid

    #214454

    Purpose

    Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    PM-miniTurbo-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPyCAG-Nanog(K140A)-IP

    Plasmid

    #62998

    Purpose

    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K140A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Lysine 140 (141 AA in Mouse Protein) to A…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p663-UBC-V5-BioID-STOP_IDG-K

    Plasmid

    #158163

    Purpose

    Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-BioID
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lazarus Gata 3 flag

    Plasmid

    #80135

    Purpose

    retroviral expression of Gata3-Flag

    Depositor

    Ellen Rothenberg

    Insert

    GATA binding protein 3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL2316

    Plasmid

    #137063

    Purpose

    loxP (empty) expression cassette: n-terminal GFP

    Depositor

    Jeremy Mottram

    Article

    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

    Type

    Empty backbone

    Use

    Leishmania floxed gene expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPyCAG-Nanog(L122A)-IP

    Plasmid

    #62992

    Purpose

    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog L122A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Leucine 122 (123 AA in Mouse Protein) to …

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQCXP CMV/TO RasV12 (w364-1)

    Plasmid

    #22268

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine (G12V)

    Promoter

    Available since

    Feb. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pResQ shFEN3 3XF-FEN1 D181A

    Plasmid

    #17753

    Depositor

    Sheila Stewart

    Insert

    Flap Endonuclease I (FEN1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Triple Flag

    Expression

    Mammalian

    Mutation

    D181A

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

36. p667-UBC-hcRed-V5-TurboID_IDG-K

    Plasmid

    #135238

    Purpose

    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    hcRed-V5-TurboID
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPyCAG-Nanog(Y136A)-IP

    Plasmid

    #62995

    Purpose

    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog Y136A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 136 (137 AA in Mouse Protein) to…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid

    #73707

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    T231A/S232A

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

39. BC-GPRC

    Plasmid

    #44199

    Depositor

    Lei Xu

    Insert

    human GPR56 C-terminal fragment (ADGRG1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    C-terminal fragment of GPR56 starting at amino a…

    Promoter

    LTR

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid

    #73702

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451

    Promoter

    CMV/tet

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pPyCAG-Nanog(K137A)-IP

    Plasmid

    #62996

    Purpose

    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K137A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Lysine 137 (138 AA in Mouse Protein) to A…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPyCAG-Nanog(Y119A)-IP

    Plasmid

    #62991

    Purpose

    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog Y119A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 119 (120 AA in Mouse Protein) to…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pB-T-PAF-Clu CC4

    Plasmid

    #215389

    Purpose

    High expression of the Clusterin(S161R) mutant for purification.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CLU (CLU Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    p.Ser161Arg

    Promoter

    tetO

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTetON-rHUH

    Plasmid

    #235192

    Purpose

    To express rHUH protein in mammalian cells

    Depositor

    Alice Ting

    Article

    Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

    Insert

    rHUH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LT3GEPIR-NEK1shRNA1

    Plasmid

    #220538

    Purpose

    To inducibly knockdown NEK1 expression

    Depositor

    Sean Lee

    Insert

    NEK1 shRNA1 (NEK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LT3GEPIR-CDK1shRNA1

    Plasmid

    #220569

    Purpose

    To inducibly knockdown CDK1 expression

    Depositor

    Sean Lee

    Insert

    CDK1 shRNA1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LT3GEPIR-CDK1shRNA2

    Plasmid

    #220570

    Purpose

    To inducibly knockdown CDK1 expression

    Depositor

    Sean Lee

    Insert

    CDK1 shRNA2 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. p663-UBC-ER-miniTurbo-V5_IDG-K

    Plasmid

    #214452

    Purpose

    Gateway destination clone of ER-miniTurbo (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-miniTurbo-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. MSCV-P C-FlagHA HPV16 E7 H73A

    Plasmid

    #191547

    Purpose

    Protein expression of HPV16 E7 H73A

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    H73A

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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