We narrowed to 18,548 results for: Mut

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pLIGFP_W220F

Plasmid

#46772

Purpose

Protein overexpression with the modified LacIWF repressor

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI_W220F (lacI )

Mutation

Tryptophan 220 replaced by Phenylalanine

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBA707

Plasmid

#186238

Purpose

KanR-BBR1 entry vector for HR donor

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRGD-GmR

Plasmid

#74106

Purpose

Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aacC1

Use

Bacterial vector based on pmb1 replicon

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-p2X

Plasmid

#75287

Purpose

Expresses proteins in the periplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pRGD-SmR

Plasmid

#74109

Purpose

Plasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aadA

Use

Bacterial vector based on pmb1 replicon

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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XE240 Fz1-ER-GFP

Plasmid

#16815

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER-GFP

Use

Xenopus expression

Tags

gfp

Mutation

F36M in FKBP

Available Since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
EBV BGLF4 KD

Plasmid

#26692

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

BGLF4

Tags

HA

Expression

Mammalian

Mutation

Kinase deficient mutant: Lysine 102 mutated to I…

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
pND004

Plasmid

#200950

Purpose

MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12D

Plasmid

#179656

Purpose

KRAS active mutant (G12D mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12D

Expression

Mammalian

Mutation

G12D

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+hSpCas9

Plasmid

#51815

Purpose

Encode hSpCas9 for in vitro transcription with Sp6

Depositor

Masato Kinoshita

Article

Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

Insert

humanized S.pyogenes Cas9

Use

CRISPR; Medaka, zebrafish

Tags

3xFLAG

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(Trp1)

Plasmid

#49459

Purpose

overespression of Flp step-arrest mutant in yeast, TRP1 selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBHt2

Plasmid

#104175

Purpose

Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum

Depositor

Seogchan Kang

Article

Mullins et al Phytopathology. 2001 Feb;91(2):173-80. d

Type

Empty backbone

Use

Fungal expression

Promoter

none

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pYOY152

Plasmid

#13987

Depositor

Linda Wordeman

Article

Ovechkina et al J Cell Biol. 2002 Nov 25. 159(4):557-62.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLG166

Plasmid

#124366

Purpose

Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Ppen

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Hs.HRAS

Plasmid

#83181

Purpose

Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLO87

Plasmid

#91726

Purpose

mycobacterial expression of mCherry from Hsp60 promoter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Insert

hsp60 promoter

Use

Mycobacterial expression

Tags

mCherry

Promoter

hsp60

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Expression

Worm

Promoter

U6 promoter

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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