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Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-EGFP
Plasmid#163609PurposeExpresses EGFP Flp-dependently in mammalian cellsDepositorInsertEgfp
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
dGFP-Granuphilin
Plasmid#170868PurposeMammalian expression of dGFP-Granuphilin.DepositorInsertSynaptotagmin-like 5 (SYTL5 Human)
UseTagsdEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_ReBphP-PCM_IRES_sfGFP
Plasmid#166917PurposeExpress ReBphP-PCM and sfGFPDepositorInsertsReBphP-PCM
sfGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_GFP_hTLN2-FL-LBD
Plasmid#164841PurposeExpression of GFP tagged FL human talin2 mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis10-GFPExpressionMammalianMutationLipid binding deficient mutationsPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 AURKA 1-30
Plasmid#157756PurposeExpression of AURKA Nter (aa1-30):GFP in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationThe first 30 amino acids of AURKA were clonedPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-sfGFP
Plasmid#162688PurposepFE vector control expressing sfGFPDepositorInsertsfGFP
UseTagsC terminal 6x His tagExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-CD8a(7A)
Plasmid#162498PurposeExpresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.DepositorInsertCD8a with a mutated stem region (CD8A Human)
UseTagsGFPExpressionMammalianMutationThe five threonine and two serine residues are mu…PromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-2xTstem(5A)-GFP
Plasmid#162504PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.DepositorUseTagsGFPExpressionMammalianMutationOne Tac stem region is inserted within ST6Gal1 an…PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem(5A)-GFP
Plasmid#162502PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe mutated Tac stem region is inserted within ST…PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-2xTstem-GFP
Plasmid#162503PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.DepositorUseTagsGFPExpressionMammalianMutationOne Tac stem region is inserted within ST6Gal1 an…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem-GFP
Plasmid#162501PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe Tac stem region is inserted within ST6Gal1PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tac(5A)-GFP
Plasmid#162495PurposeExpresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac(5A)
Plasmid#162493PurposeExpresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(2)
Plasmid#163428PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT
Plasmid#155248Purposefor mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only