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p4949 pLPCX-His-Xpress NLS hBrd4 CTD

Plasmid

#14459

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Use

Retroviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pAG416GAL-ccdB-mRuby3

Plasmid

#166139

Purpose

Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

mRuby3

Expression

Yeast

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

Plasmid

#108484

Purpose

inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.1.mKO2

Plasmid

#85209

Purpose

TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RASEFIR-HA

Plasmid

#46863

Purpose

expresses mouse IL-17RA with SEFIR deletion

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-delta-SEFIR (Il17ra Mouse)

Tags

3x HA

Expression

Mammalian

Mutation

Deletion of SEFIR domain (AA 394-485)

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

Plasmid

#190898

Purpose

Entry vector for multiple sgRNA cloning

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

Plasmid

#194461

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRFcT

Plasmid

#44573

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF2 where TRFH domain (aa42-241) has been replac…

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

Plasmid

#194463

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-hspB2

Plasmid

#63093

Purpose

Expression of HSPB2 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb2 (HSPB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
GFP-AuroraB L154G/H250Y

Plasmid

#108492

Purpose

expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMADM-alpha

Plasmid

#36890

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, deleted…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCR3-Flag-AuroraB KD (K106R)

Plasmid

#108488

Purpose

expression of Flag-AuroraB kinase death (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

Flag

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

Plasmid

#194459

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a del.SHD

Plasmid

#40036

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a del.SHD (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted SHD domain (1-143)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pTol2-PcyA-IRES-HO1

Plasmid

#131787

Purpose

Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PcyA and HO1

Use

Synthetic Biology; Zebrafish tol2 transposon

Tags

MTS (Mitochondrial Targeting Signal from subunit …

Expression

Mammalian

Promoter

hsp70

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2A

Plasmid

#40038

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A domain (353-487)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

Plasmid

#14442

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
H14-MBP-SUMO-Cys-Linker-BAF_L58R

Plasmid

#101780

Purpose

Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

L58R mutation

Promoter

T5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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