162,325 results

1. CRY2olig-mCherry

   Plasmid

   #60032

   Purpose

   Expresses a fusion of CRY2PHR E490G with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2PHR E490G (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. LacI-pLacI-pIII pm

   Plasmid

   #218100

   Purpose

   Phagemid that expresses LacI, and pIII under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROP-seq-puro-v2-no-scaffold

   Plasmid

   #228521

   Purpose

   CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

   Depositor

   Jellert Gaublomme

   Article

   Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

   Inserts

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP-seq-mTurquoise2-v2-no-scaffold

   Plasmid

   #228522

   Purpose

   CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

   Depositor

   Jellert Gaublomme

   Article

   Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

   Insert

   mTurquoise2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cbh_v5 AAV-ABE C-terminal

   Plasmid

   #137178

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mCherry-CNA35

   Plasmid

   #61607

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mCherry

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mCherry and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 Pten

   Plasmid

   #59909

   Purpose

   pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-flag S127A YAP

   Plasmid

   #27370

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   S127A ( major phosphorylation site in YAP2)

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES2.1-TRP1

    Plasmid

    #11007

    Depositor

    Francisco Malagon

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Tags

    V5 and his6

    Expression

    Bacterial and Yeast

    Mutation

    ORF-TRP1 without stop codon

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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