We narrowed to 88,257 results for: Mal

1. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

   Plasmid

   #170248

   Purpose

   Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   C124S

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-RUSH-LAMP2A-mNeonGreen

   Plasmid

   #228525

   Purpose

   Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

   Depositor

   Ginny Farias

   Article

   Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

   Insert

   LAMP2 (Lamp2 Rat)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP), Streptavidin-…

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_CCNB1-P2A-Hygro_Barcode

   Plasmid

   #170218

   Purpose

   Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1-Pyl-TAG

   Plasmid

   #228784

   Purpose

   Pyl aaRS/tRNA system for mammalian TAG suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   PylRS
   PytR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH231-EF1-BLST-dCas9-VPR

   Plasmid

   #115148

   Purpose

   Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   BlastR-P2A-dCas9-VPR
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER-21-SPI1

   Plasmid

   #97039

   Purpose

   Expresses SPI1 in mammalian cells

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Spi-1 Proto-Oncogene (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet on

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

8. MO91-dCas9-AID(P182X)

   Plasmid

   #83260

   Purpose

   Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

   Depositor

   Xing Chang

   Article

   Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

   Insert

   dCas9-AID P182X
   

   Use

   Retroviral

   Tags

   3xFLAG-NLS and FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9; P182X in AID

   Promoter

   MSCV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

9. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC34

    Plasmid

    #62331

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CKIIa-stGtACR1-FusionRed

    Plasmid

    #105679

    Purpose

    Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 BMPS HK318/9EE-6xHis

    Plasmid

    #204836

    Purpose

    Mammalian expression of recombinant BMPS WT

    Depositor

    Monther Abu-Remaileh

    Article

    Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

    Insert

    CLN5 (CLN5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    HK318/9EE

    Promoter

    CMV

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. px551-mSncg-0.27kb-HA-SpCas9

    Plasmid

    #153208

    Purpose

    Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated mSncg (0.27kb)

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_NRL_P2A_Hygro_Barcode

    Plasmid

    #120469

    Purpose

    Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NRL (NRL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5 BMPS C231S-6xHis

    Plasmid

    #204834

    Purpose

    Mammalian expression of recombinant BMPS C231S

    Depositor

    Monther Abu-Remaileh

    Article

    Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

    Insert

    CLN5 (CLN5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    C231S

    Promoter

    CMV

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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