We narrowed to 5,331 results for: pcdna3.1

1. pGAL4-DBD-MCS

   Plasmid

   #145245

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA Arf6

   Plasmid

   #10834

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HA-Nedd4/2795

   Plasmid

   #11426

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D661N compared to GenBank reference sequence U966…

   Promoter

   CMV

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-CyclinF-ΔF

   Plasmid

   #32973

   Depositor

   Michele Pagano

   Article

   D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

   Insert

   CYCLIN F (CCNF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F-box mutations: L35A and P36A

   Promoter

   CMV

   Available Since

   Feb. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

5. GFP1-10-miniCMV-GFP11×11-GFP-tDeg

   Plasmid

   #185404

   Purpose

   Expresses GFP1-10 and GFP11×11-GFP-tDeg

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   GFP1-10
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CCR5

   Plasmid

   #98943

   Purpose

   Mammalian expression plasmid for untagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-KDEL

   Plasmid

   #124316

   Purpose

   Expresses ER specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Tags

   KDEL Seq

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0079 RESCUE-S

    Plasmid

    #130662

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEYFP-NR1a

    Plasmid

    #17928

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid

    #227246

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOZITX-S1

    Plasmid

    #184925

    Purpose

    Expresses the catalytic S1 subunit of OZITX (EcPltAB)

    Depositor

    Jonathan Javitch

    Article

    Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

    Insert

    codon optimized S1 subunit of OZITX
    

    Expression

    Mammalian

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-Cyto

    Plasmid

    #124314

    Purpose

    Expresses cytosolic specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Tags

    Cytoplasm specific seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Halo-MTS

    Plasmid

    #124315

    Purpose

    Expresses mitochondria specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Tags

    Mito specific seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p4B5-EGFP

    Plasmid

    #140324

    Purpose

    Detection of Dengue virus infection in live cells

    Depositor

    Alan Rothman

    Article

    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

    Insert

    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    2 conservative n.t. differences near 5' end …

    Promoter

    CMV

    Available Since

    May 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cytosolic Syk Biosensor

    Plasmid

    #125729

    Purpose

    FRET biosensor for studying SYK activities in cells

    Depositor

    Yingxiao Wang

    Article

    Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

    Insert

    SYK biosensor (ZAP70 Synthetic)
    

    Tags

    6xHistine Tag at the N-terminal.

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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