We narrowed to 2,962 results for: ER

1. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSynapsin.SF-iGluSnFR.A184V

   Plasmid

   #106175

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V

   Promoter

   hSynapsin

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet-TFIIF

   Plasmid

   #171082

   Purpose

   Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   RAP30 (GTF2F2 Human)
   RAP74 (GTF2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6p-1-CDK5RAP3

   Plasmid

   #204566

   Purpose

   Expresses GST-CDK5RAP3 in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. eGFP-proα1(I)

   Plasmid

   #119843

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Voltron-ST

   Plasmid

   #119034

   Purpose

   Express soma-localized Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-jYCaMP1

   Plasmid

   #135423

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Insert

   jYCaMP1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

   Plasmid

   #134645

   Purpose

   lysosome-targeted Sac1 catalytic domain wild type

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   Sac1 catalytic domain (77-520 aa) (SACM1L Human)
   

   Use

   Lentiviral

   Tags

   FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

   Expression

   Mammalian

   Mutation

   contains only amino acids 77-520 (Please see depo…

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid

    #134653

    Purpose

    lysosome-targeted Sac1 catalytic domain C389S

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    

    Use

    Lentiviral

    Tags

    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

    Expression

    Mammalian

    Mutation

    C389S and contains only amino acids 77-520 (Pleas…

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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