We narrowed to 38,405 results for: ANT

1. pBITE-EGFRvIII-F271-OKT3

   Plasmid

   #190682

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLP712

   Plasmid

   #203741

   Purpose

   Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   OsTIR1
   

   Use

   Candida albicans integrating vector

   Tags

   3xMyc

   Expression

   Mutation

   Codon 74 changed from Phe to Ala (F74A)

   Promoter

   ACT1 (C. albicans)

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. dpYPQ230-D156R-ABE8

   Plasmid

   #195569

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. dpYPQ230-D156R-CBE2

   Plasmid

   #195368

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-L1T3D

   Plasmid

   #33276

   Depositor

   Terence Dermody

   Article

   Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

   Insert

   L1 (L1, L1 Orthoreovirus T3D)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Jan. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mTFP1-Vinculin-23

   Plasmid

   #55516

   Purpose

   Localization: Focal Adhesions, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-Myc3-Cul3 delta N41

   Plasmid

   #21590

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Residues 34-74 deleted from N terminus.

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pMH-Cas9-gate

    Plasmid

    #113742

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. gWiz_BM40-vGAT-VL-hIgG1-CL

    Plasmid

    #218437

    Purpose

    mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-VL-hIgG1-CL
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mH2A1.2-CT-GFP

    Plasmid

    #45169

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDx_mRuby3-SYFP2

    Plasmid

    #189758

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mRuby3-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCyterm-mScarlet-I3-NCwt_N1

    Plasmid

    #189779

    Purpose

    Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    Cyterm-mScarlet-I3-NCwt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYDS2.0_AT118-L

    Plasmid

    #222847

    Purpose

    Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. dpYPQ230-D156R-ABE4

    Plasmid

    #195370

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GGGGS)x6-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLPP5

    Plasmid

    #209967

    Purpose

    Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_sppQ)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPP6

    Plasmid

    #209968

    Purpose

    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter Dropout (sfGFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGG-cTP-TurboID-GFP

    Plasmid

    #209403

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    Suzanne Jackowski, T. J. Kappock

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMP71-tCD19-SIINFEKL

    Plasmid

    #174601

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane bound CD19, SIINFEKL (ovalbumin epitope)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMRXIP-GFP-STX17ΔNTD

    Plasmid

    #89941

    Purpose

    Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

    Insert

    human syntaxin17 lacking N-terminal domain (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    deleted amino acids 1-161

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. hLOXL2-S300P

    Plasmid

    #200061

    Purpose

    LOXL2 mutant

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S300P mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD02_CBASS_Escherichia_coli_703128

    Plasmid

    #224394

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli 703128

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnD_4TM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBAD02_CBASS_Escherichia_coli_BWH59

    Plasmid

    #224393

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli BWH59

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    2TM_EcCdnD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDGE479

    Plasmid

    #153270

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTrex-Neo-tdTomato

    Plasmid

    #47975

    Purpose

    Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    tdTomato
    

    Use

    Trypanasoma cruzi expression

    Tags

    Expression

    Mutation

    Promoter

    T. cruzi rRNA

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ePPEmax*

    Plasmid

    #205244

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMpGE006

    Plasmid

    #108722

    Purpose

    Cas9 expression in M.polymorpha

    Depositor

    Shigeo Sugano, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Insert

    Atco-Cas9-Pea3ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEFpro

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge