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Addgene

We narrowed to 8,371 results for: BLI;

Showing: 7361 - 7400 of 8371 results
  1. pQC V5 HuR.wt IRES Puro

    Plasmid
    #110386
    Purpose
    γ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA5/FRT/TO/Flag-mKO2-HuR.DelHNS

    Plasmid
    #110373
    Purpose
    Mammalian expression of mKO2-HuR (DelHNS) with FLAG tag, Flp-In T-Rex system
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Tags
    FLAG and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid
    #232341
    Purpose
    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-48 (indicated as Mito-signal in UniPro…
    Promoter
    CMV
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid
    #214468
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. GL4.10 E2E1- Prg4 promoter-luciferase

    Plasmid
    #195035
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pGL4.10 E3E2E1-Prg4 promoter-luciferase

    Plasmid
    #195036
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

    Plasmid
    #195037
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    Insert
    Bcl-2-interacting killer (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid
    #158616
    Purpose
    B6J Id1-LSL-DTA allele targeting vector, low copy number
    Depositor
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…
    Promoter
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. GFP-MIOX only

    Plasmid
    #166683
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    GFP-MIOX
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Insert
    UDH
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. psiCHECK2 3'UTR KGA

    Plasmid
    #110402
    Purpose
    Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pX330.puro sgRNA KGA Stop1

    Plasmid
    #110405
    Purpose
    CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

    Plasmid
    #110408
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. p1A CbbL-

    Plasmid
    #162693
    Purpose
    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk
    Depositor
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    CbbL K194M (inactive rubisco)
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7361 - 7400 of 8371 results