We narrowed to 9,075 results for: sgRNA

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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Publication

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9 CFP

Plasmid

#155278

Purpose

Lentiviral Cas9 expression with CFP marker

Depositor

Martin Carroll

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

spCas9

Expression

Mammalian

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_AAVS1

Plasmid

#141212

Purpose

pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control

Depositor

Rory Johnson

Insert

gRNA

Use

Lentiviral

Expression

Mammalian

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Promoter

Bacteria

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX458 V3

Plasmid

#226957

Purpose

CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDECKO_GFP_AAVS1_186

Plasmid

#159091

Purpose

targeting, non-essential locus control

Depositor

Rory Johnson

Insert

gRNA

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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B-SpCas9-HF1

Plasmid

#126762

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Pdg459-eSp(1.1) V3

Plasmid

#226965

Purpose

CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_835

Plasmid

#245327

Purpose

Cas9 CRISPRko positive control guide; targets CD59

Depositor

John Doench, David Root

Insert

sgRNA CD59 (CD59 Synthetic)

Use

Lentiviral

Mutation

None

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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ttAtCas12a+int

Plasmid

#182384

Purpose

Gives very high editing efficiency in barley. Also works in Brassica oleracea

Depositor

Wendy Harwood

Publication

Lawrenson et al bioRxiv 2022.04.28.489853

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LbCas12a coding sequence with D156R and Arabidopsis introns

Use

CRISPR

Mutation

D156R + 8 Arabidopsis introns added

Promoter

None

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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PX458 eSp(1.1) V3

Plasmid

#226963

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pK2-J11

Plasmid

#218224

Purpose

Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK4-J11

Plasmid

#218225

Purpose

Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK6-J11

Plasmid

#218226

Purpose

Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK8-J11

Plasmid

#218227

Purpose

Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-AmCyan

Plasmid

#138481

Purpose

For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AmCyan

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK2-K3

Plasmid

#218221

Purpose

Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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