We narrowed to 10,760 results for: ESP

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KIF2A D8.2 gRNA

Plasmid

#90721

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF2A (Guide Designation D8.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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CEP55 B12.2 gRNA

Plasmid

#90623

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Nov. 15, 2017

Availability

Academic Institutions and Nonprofits only

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CEP55 B11.2 gRNA

Plasmid

#90622

Purpose

3rd generation lentiviral gRNA plasmid targeting human CEP55

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CEP55 (Guide Designation B11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pD765 FDFT1 3'UTR

Plasmid

#105765

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

FDFT1 3'UTR (FDFT1 Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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ATAD2 D8.3 gRNA

Plasmid

#90539

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATAD2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ATAD2 (Guide Designation D8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F10.4 gRNA

Plasmid

#90941

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F9.4 gRNA

Plasmid

#90940

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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POT1 E3.4 gRNA

Plasmid

#90842

Purpose

3rd generation lentiviral gRNA plasmid targeting human POT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

POT1 (Guide Designation E3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES

Plasmid

#175811

Purpose

The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SMC3 A8.4 gRNA

Plasmid

#90897

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 A7.4 gRNA

Plasmid

#90896

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H8.1 gRNA

Plasmid

#90895

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H8.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H7.1 gRNA

Plasmid

#90894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H7.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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KIF11 F5.3 gRNA

Plasmid

#90716

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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RNASEH2A B10.3 gRNA

Plasmid

#90879

Purpose

3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RNASEH2A (Guide Designation B10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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FOXM1 C12.2 gRNA

Plasmid

#90691

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGMC00013

Plasmid

#172525

Purpose

sgRNA against mouse Mr1

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Mr1 (Mr1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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DHCR7 B6.3 gRNA

Plasmid

#90651

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B6.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DHCR7 B5.3 gRNA

Plasmid

#90650

Purpose

3rd generation lentiviral gRNA plasmid targeting human DHCR7

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DHCR7 (Guide Designation B5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI (mutant)

Plasmid

#206322

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag-mutated IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein (mutant version)

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CCNA2 B2.1 gRNA

Plasmid

#90575

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNA2 (Guide Designation B2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CCNA2 B1.1 gRNA

Plasmid

#90574

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNA2 (Guide Designation B1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-sfGFP-MiniWhite

Plasmid

#165910

Purpose

Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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TOP2A G11.2 gRNA

Plasmid

#90914

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2516

Plasmid

#91084

Purpose

Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

At7SL

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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ANLN A2.2 gRNA

Plasmid

#90531

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-KD-mAbl

Plasmid

#69028

Purpose

Retorviral vector encoding Kinase Defective mouse c-Abl

Depositor

Jean Wang

Article

Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

Insert

mouse c-Abl Type VI - kinase defective

Use

Retroviral

Tags

FLAG tag inserted at Internal SalI site (2934 bp)

Expression

Mammalian

Mutation

The KD (kinase defective) mutation is at Lysine 2…

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only