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pCMV_Xph15-GCaMP6f

Plasmid

#135538

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

Depositor

Matthieu Sainlos

Publication

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

GCaMP6f

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre-Y324F

Plasmid

#122961

Purpose

Catalytically inactive mutant of PA-Cre

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre-Y324F

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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TALEN1297

Plasmid

#32279

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Publication

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9

Plasmid

#169335

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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891 pFastBac GST PTEN

Plasmid

#10937

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Insect

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pDR111_Bgal no NLS

Plasmid

#188397

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Publication

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

Phyper spank

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP5

Plasmid

#160088

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

Depositor

Pier Pandolfi

Publication

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB 35s:dCas:BRD:tNos (GB1172)

Plasmid

#75401

Purpose

Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

dCas9:BRD

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

35S

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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PX400 Campylobacter lari CF89-12 Cas9

Plasmid

#68336

Purpose

Campylobacter lari CF89-12 Cas9

Depositor

Feng Zhang

Insert

ClCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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1438 pSG5L Flag HA PTEN G129R

Plasmid

#10775

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

G129R

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Expression

Mammalian

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Publication

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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nSaCas9 I744 miniABEMax V82G

Plasmid

#135379

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

Depositor

Alex Hewitt

Publication

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 I744 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAMP-CY1_NF1 T2 (+minintr)

Plasmid

#119205

Purpose

transfer vector

Depositor

Helen Morrison

Publication

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Publication

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Publication

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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