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pTol2CG2-QUASM1:mScarletNLS-SV40pA

Plasmid

#155124

Purpose

Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:mScarletNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightG2

Plasmid

#221670

Purpose

Expresses nLightG2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightG2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR2

Plasmid

#221671

Purpose

Expresses nLightR2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightR2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGAPDH::sNluc1

Plasmid

#187221

Purpose

A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Overexpression

Promoter

GAPDH

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Native promoter dCas9

Plasmid

#113656

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

Native promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

S. pyogenes Cas9 native promoter

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightR2-control

Plasmid

#221675

Purpose

Expresses nLightR2-control in neurons

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightR2-control

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightG2-control

Plasmid

#221674

Purpose

Expresses nLightG2-control in neurons

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightG2-control

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Neurospora crassa, Zebrafish, Budding Yeast)

Use

Zebrafish expression

Promoter

hsp70l

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Promoter

acta1

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TAP_FAM86A

Plasmid

#85116

Purpose

mammalian expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Tags

CBP-SBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2

Plasmid

#174491

Purpose

bacterial expression of human SEPT2

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAF197

Plasmid

#135930

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

CTLA4 fused to IgG Fc and to mCherry

Use

Transposon

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/T645A

Plasmid

#74936

Purpose

Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

T645A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pnEA-vH_His-TEV-SEPT2_SEPT6

Plasmid

#174497

Purpose

bacterial co-expression of human SEPT2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT6_SEPT7-TEV-Strep

Plasmid

#174499

Purpose

bacterial co-expression of human SEPT6 and of human SEPT7

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN6 (SEPTIN6 Human)
SEPTIN7 (SEPTIN7 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT

Plasmid

#82892

Purpose

Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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