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pAc5.1B-lambdaN-HA-DmEDC3_441-680_D

Plasmid

#146130

Purpose

Insect Expression of DmEDC3_441-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_441-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61A_D

Plasmid

#146131

Purpose

Insect Expression of DmEDC3-D61A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61A (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61K_D

Plasmid

#146132

Purpose

Insect Expression of DmEDC3-D61K

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61K (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-N44A_D

Plasmid

#146135

Purpose

Insect Expression of DmEDC3-N44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-N44A (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-RB_B

Plasmid

#145977

Purpose

Insect Expression of DmAGO1-RB

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmAGO1-RB (AGO1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3_A

Plasmid

#145895

Purpose

Insect Expression of DmNot3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot3 (Not3 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A

Plasmid

#145896

Purpose

Insect Expression of DmAGO1-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F2V2 (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F594V_A

Plasmid

#145897

Purpose

Insect Expression of DmAGO1-F594V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F594V (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A

Plasmid

#145899

Purpose

Insect Expression of DmAGO1-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmXRN1_1323-1355_N

Plasmid

#147052

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355 (pcm Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

Plasmid

#145898

Purpose

Insect Expression of DmAGO1-F629V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F629V (AGO1 Fly)

Expression

Insect

Mutation

V315A, F629V, H913R - variation with 2 non silent…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159829

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Plasmid

#159838

Purpose

Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Expression

Worm

Mutation

Not applicable

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575_AG

Plasmid

#148752

Purpose

Mammalian Expression of HsNot4_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575 (CNOT4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRRG245-Ras-related

Plasmid

#99079

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ras-related (also known as h.18.4a) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pVH003

Plasmid

#80397

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold - Venus YFP
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

Fused by GS linker to LZx domain and Venus has N-…

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pES006 (pTet-qacR 2/pQacA-deGFP)

Plasmid

#69035

Purpose

reporter with qacR 2 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA

Plasmid

#185715

Purpose

AAV expression of GFP and human α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Promoter

hSyn

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-mSNCA

Plasmid

#185714

Purpose

AAV expression of GFP and mouse α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (Snca Mouse)

Use

AAV

Promoter

hSyn

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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