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Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK1010 (pSL25)
Plasmid#191005PurposeExpresses blue chromoprotein on a broad host range RP4 origin plasmidDepositorInsertblue chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK1
Plasmid#122672PurposeExpresses mouse PCSK1 in mammalian cellsDepositorInsertproprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3362
Plasmid#144838PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYOG (MYOG Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV PGK1-3xHA
Plasmid#220913PurposeExpresses PGK1-3xHADepositorInsertPGK1-3xHA (PGK1 Human)
UseAffinity Reagent/ AntibodyTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniE-DBCO
Plasmid#181985PurposeUsed to label proteins with the MiniR genetically encoded tagDepositorInsertMiniE-DBCO
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only