159,557 results

1. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hBACCS2-IRES-mCherry

   Plasmid

   #72892

   Purpose

   Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   hBACCS2-IRES-mCherry
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInt-ts

   Plasmid

   #66076

   Purpose

   The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   lambda integrase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP2-96-merTetO-EFS-BLaR

   Plasmid

   #118713

   Purpose

   Contains an optimized 96-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFD6

   Plasmid

   #73915

   Purpose

   tissue-specific multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   :tRNA:gRNA:tRNA:gRNA:tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS-DSCP

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hChR2(H134R)-EYFP

   Plasmid

   #26973

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-mitoGshF-blasticidin

   Plasmid

   #210314

   Purpose

   Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) NAPstar3b

   Plasmid

   #232736

   Purpose

   Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstar3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBPGAL80Uw-6

    Plasmid

    #26236

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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