We narrowed to 3,917 results for: LYC

1. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAd-M3cherry

   Plasmid

   #61041

   Purpose

   This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

   Depositor

   Qiao Zhou

   Article

   Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

   Inserts

   Ngn3 (Neurog3 Mouse)
   pdx1 (Pdx1 Mouse)
   Mafa (Mafa Mouse)
   monomeric cherry
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_SARS2_omicron_BA.1

   Plasmid

   #180375

   Purpose

   Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   Spike (omicron BA.1 variant) (S SARS-CoV-2)
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   c terminal D18

   Promoter

   CMV

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS SARS-CoV-2 BA.2.12.1 Spike

   Plasmid

   #186032

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

   Depositor

   Marceline Côté

   Article

   Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

   Insert

   SARS-CoV-2 BA.2.12.1 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the BA.2 mutations (T19I, LPPA24S, G142D…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3_SARS2_omicron BA.2

   Plasmid

   #183700

   Purpose

   expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   Spike (BA.2) (S SARS-CoV-2)
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   c-terminal 18 aa deletion

   Promoter

   CMV

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

   Plasmid

   #179905

   Purpose

   Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

   Insert

   SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

   Promoter

   CAG

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3_SARS2_BQ.1.1

   Plasmid

   #194493

   Purpose

   expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (BQ.1.1) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R346T, K444T, N460K and c-terminal 18aa del on th…

   Promoter

   CMV

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3_SARS2_XBB

   Plasmid

   #194494

   Purpose

   expressing SARS-CoV-2 XBB spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (XBB) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

   Promoter

   CMV

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-GSK3A

    Plasmid

    #23510

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    GSK3A (GSK3A Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAP50-COMP-blac-flag-his

    Plasmid

    #110956

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    secreted acid phosphatase (GAP50) (PF3D7_0918000 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_SARS2_BA.4/5

    Plasmid

    #186810

    Purpose

    pseudovirus plasmid expressing BA.4 and BA.5 spike protein

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    43740568 (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HY69-70del, L452R, F486V, Q493R, on the basis of …

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-DGKA

    Plasmid

    #23837

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DGKA (DGKA Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. MAEBL-COMP-blac-flag-his

    Plasmid

    #110963

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid

    #184830

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.3_SARS2_BA.2.12.1

    Plasmid

    #186809

    Purpose

    pseudovirus plasmid that expresses spike of BA.2.12.1

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (BA.2.12.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L452Q, S704L on the basis of BA.2, c-terminal 18 …

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid

    #184831

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Spike Display_B.1.1.7

    Plasmid

    #172735

    Purpose

    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. GRP94-COMP-blac-flag-his

    Plasmid

    #111008

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge