We narrowed to 75,864 results for: Rest

1. pnos-Fok1:dCas9-nos

   Plasmid

   #62210

   Purpose

   Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Insert

   Fok1-dCas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RAB27B

   Plasmid

   #25545

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RAB27B (RAB27B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pRSII306

   Plasmid

   #35441

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRIOBP-TwinStrepII

   Plasmid

   #83201

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TRIOBP (Triobp Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-NT-3-mCherry

   Plasmid

   #190201

   Purpose

   To express the mouse NT-3 tagged with the FP mCherry

   Depositor

   Pantelis Tsoulfas

   Insert

   NT-3
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   The codon were optimized for better expression

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57_beta-globin-5'UTR_Nanoluc

   Plasmid

   #219989

   Purpose

   betaglobin-5'UTR-NanoLuc mRNA synthesis

   Depositor

   Simon Alberti

   Insert

   betaglobin-5'UTR-NanoLuc (HBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sfCherry11-620W-PTPN22

   Plasmid

   #195386

   Purpose

   Mammalian expression of a human PTPN22 mutant (620W) with sfCherry(11) tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Tags

   sfCherry(11)

   Expression

   Mammalian

   Mutation

   R620W (rs2476601)

   Promoter

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH385-GST-TEV-SUP35

   Plasmid

   #29744

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-Basic-OPN-1200

   Plasmid

   #11996

   Depositor

   Steven Johnson

   Insert

   Osteopontin (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The following construct contains the OPN promoter…

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-EGFP-DmRRP40

    Plasmid

    #65797

    Purpose

    expresses EGFP-tagged RRP40 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    RRP40 (Rrp40 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPro24

    Plasmid

    #17805

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RAB26

    Plasmid

    #25528

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB26 (RAB26 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pKW08-Lx-Int

    Plasmid

    #25015

    Depositor

    Brian Robertson

    Article

    Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

    Insert

    pKW08-Lx-Int
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mouse ptc probe M2-3

    Plasmid

    #58701

    Purpose

    makes mouse ptc probe for in situ hybridization

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Bacterial

    Mutation

    only EcoRI fragment (5' end)

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RAB31

    Plasmid

    #25552

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB31 (RAB31 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK615

    Plasmid

    #71699

    Purpose

    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPD708 DsRed-Express2-ZF21

    Plasmid

    #138795

    Purpose

    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.

    Depositor

    Joshua Leonard

    Insert

    DsRed-Express2-ZF21
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMN234

    Plasmid

    #32108

    Depositor

    Michael Niederweis

    Article

    Stephan et al Gene. 2004 Dec 8;343(1):181-90.

    Insert

    flpe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    imyc

    Available since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RAB30

    Plasmid

    #25542

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB30 (RAB30 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pRL-TK CXCR4 6x

    Plasmid

    #11308

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    6 bulged bind sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBMTBX-1

    Plasmid

    #26072

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LHP398 - Ub-K48R/K63R

    Plasmid

    #32167

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K48R and K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-MK2

    Plasmid

    #30187

    Depositor

    Bruce Tempel

    Insert

    MK2 (Kcna2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTYB2-eIF1

    Plasmid

    #37217

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pflaG

    Plasmid

    #45477

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    flaA

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

37. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Use

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

38. DUSP7

    Plasmid

    #27976

    Depositor

    Igor Astsaturov

    Insert

    DUSP7 (DUSP7 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

39. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

    Plasmid

    #194246

    Purpose

    Expresses tagBFP2

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    tagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    May 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. RAB2B

    Plasmid

    #25534

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB2B (RAB2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

42. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. RAB9B

    Plasmid

    #25625

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB9B (RAB9B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

44. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCSegfpcherry

    Plasmid

    #22465

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    EGFP-mCherry
    

    Use

    Injection construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Tobacco Etch Virus NIa protease (GB0420)

    Plasmid

    #160553

    Purpose

    pUPD_NIaPro(TEV)

    Depositor

    Diego Orzaez

    Insert

    Tobacco Etch Virus NIa protease
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. EMSV-MRF4 (puro)

    Plasmid

    #14713

    Depositor

    Michael Rudnicki

    Article

    Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

    Insert

    MRF4 (Myf6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2007

    Availability

    Academic Institutions and Nonprofits only

49. CbSBS-Flag/TRRAP(S722F)

    Plasmid

    #32105

    Depositor

    Yardena Samuels

    Insert

    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    

    Use

    Tags

    Flag 1x

    Expression

    Mammalian

    Mutation

    Changes serine 722 to Phe

    Promoter

    CMV

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

50. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Use

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge