We narrowed to 13,583 results for: TIM

1. PP74

   Plasmid

   #78944

   Purpose

   Supplemental Figure 3. Integration Site 1.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HPGD

   Plasmid

   #39154

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HPGD (HPGD Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAL-pgi_NT

   Plasmid

   #74067

   Purpose

   pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pgi (NT)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHN873

   Plasmid

   #178829

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A (AR Human)
   

   Use

   Tags

   MBP, His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFF145

   Plasmid

   #61446

   Purpose

   aTc-inducible Beta recombinase

   Depositor

   Timothy Lu

   Article

   Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

   Insert

   Beta recombinase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pTetO

   Available since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUap97GW (C4)

   Plasmid

   #74020

   Purpose

   lentiviral expression of SAP97alpha-EGFP fusion

   Depositor

   Weifeng Xu, Oliver Schluter, Robert Malenka

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 alpha
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKG113

   Plasmid

   #63073

   Purpose

   Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-T (CENPT Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   E. coli codon optimized. G217R mutation compared …

   Promoter

   Available since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Actinin-C-stFRET

   Plasmid

   #61104

   Purpose

   Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

   Depositor

   Fred Sachs

   Article

   Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

   Insert

   α-actinin-stFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCMV_SABV_GPC

    Plasmid

    #207305

    Purpose

    Mammalian expression of Sabia virus GPC protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SABV_GPC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB67

    Plasmid

    #47947

    Purpose

    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFF714

    Plasmid

    #61449

    Purpose

    IPTG-inducible SCRIBE_galK(OFF)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_galK(OFF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMB63

    Plasmid

    #47945

    Purpose

    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFF706

    Plasmid

    #61448

    Purpose

    Light-inducible SCRIBE_kanR(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_kanR(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pDawn

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p70rg

    Plasmid

    #17827

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pAWp9

    Plasmid

    #73851

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B1979

    Plasmid

    #59181

    Purpose

    Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    Blasticidin S resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rpl-28

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pALPS rtTA3_V14

    Plasmid

    #101350

    Purpose

    Codon optimized rtTA3 used in trans with Tet inducible HIV-1

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBB HA BIR 1-2-3

    Plasmid

    #25692

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 1-350

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pYCTK019

    Plasmid

    #176723

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Ca
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEBB-HA-hILP

    Plasmid

    #25674

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pFF758

    Plasmid

    #61454

    Purpose

    IPTG-inducible msd(wt)_dRT

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd(wt)_dRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D197A D198A

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRN104

    Plasmid

    #127231

    Purpose

    35S::AFR5::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Mpdelta
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAL-pyk_T

    Plasmid

    #74070

    Purpose

    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pyk (T)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pEBB HA BIR 2

    Plasmid

    #25688

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 124-261

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pMM100

    Plasmid

    #127225

    Purpose

    AtU6::sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICSL80004

    Plasmid

    #50325

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lvl0 mKate2 CDS

    Plasmid

    #153394

    Purpose

    Far red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thaliana

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    mKate2 CDS
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HSD11B1

    Plasmid

    #39155

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD11B1 (HSD11B1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    T220I; codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pYCTK025

    Plasmid

    #176729

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Lm
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYCTK028

    Plasmid

    #176732

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Tp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. XX01CAPN2A-c001

    Plasmid

    #175492

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    CAPN2 / CAPNS1 (CAPN2 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

    Plasmid

    #81247

    Purpose

    Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEF1-puro/hpb1

    Plasmid

    #59976

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 headpiece (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pEBB HA BIR 1

    Plasmid

    #25687

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 1-124 (BIR 1)

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pDUET-ctCPR-sAMO

    Plasmid

    #20111

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and codon optimized amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

38. PP75

    Plasmid

    #78945

    Purpose

    Supplemental Figure 3. Integration Site 2.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    Integration Site 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBW-m4133256

    Plasmid

    #152133

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYCTK009

    Plasmid

    #176713

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Eremothecium cymbalariae (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ec
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYCTK020

    Plasmid

    #176724

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Ec
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYCTK030

    Plasmid

    #176734

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Ca
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pYCTK031

    Plasmid

    #176735

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    bar1Ec
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pYCTK008

    Plasmid

    #176712

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ca
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEBB HA BIR 1-2

    Plasmid

    #25690

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 1-261 (BIR 1&2)

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

47. pFF749

    Plasmid

    #61452

    Purpose

    IPTG-inducible msd_kanR(ON)_dRT

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd_kanR(ON)_dRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D197A D198A

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pNR186

    Plasmid

    #79552

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. GRIP1

    Plasmid

    #38884

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GRIP1 (GRIP1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    C184S; codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pAWp7-12

    Plasmid

    #72744

    Purpose

    pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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