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nLbCas12a

Plasmid

#213745

Purpose

For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

nLbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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p415-GalL-Cas9-CYC1t

Plasmid

#43804

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

GalL

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX

Plasmid

#129285

Purpose

Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

Fluorescent reporter mCherry fused to CAAX polybasic sequence

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMDJ39 - Peft-3 Cre (PATCs)

Plasmid

#191381

Purpose

High efficiency CRE recombinase expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Peft-3 Cre tbb-2 UTR

Expression

Worm

Promoter

Peft-3

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Expression

Bacterial

Mutation

NA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Cre

Plasmid

#170367

Purpose

AAV vector expressing Cre under human synapsin promotor

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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plRL118

Plasmid

#163632

Purpose

Renilla luciferase reporter plasmid; Giles integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

Luciferase

Expression

Bacterial

Promoter

Mycobacterial Optimized Promoter (MOP)

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Plasmid

#209728

Purpose

Expresses cytosolic high affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-SESN2

Plasmid

#34820

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

sestrin 2 (SESN2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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