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Showing: 9351 - 9400 of 167150 results

  1. pTSHZ1.1.0-gDNA

    Plasmid
    #132434
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132434-plasmid-map-sequence-id-266216

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  2. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  3. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  4. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  5. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  6. C102m

    Plasmid
    #121006
    Purpose
    MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).
    Depositor
    Richard Murray
    Insert
    treRT with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121006-plasmid-map-sequence-id-236159

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  7. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  8. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  9. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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  10. P12m

    Plasmid
    #120938
    Purpose
    MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pLasTetO
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120938-plasmid-map-sequence-id-256615

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  11. P15m

    Plasmid
    #120939
    Purpose
    MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinTetO
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120939-plasmid-map-sequence-id-238035

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  12. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  13. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  14. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  15. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  16. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126880-plasmid-map-sequence-id-249730

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  17. pcDNA3-Flag-RNF125 Ring Mutant

    Plasmid
    #122046
    Purpose
    express RNF125 RM
    Depositor
    Ze'ev Ronai
    Insert
    RNF125 RM (RNF125 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ring mutant C37A, C40A
    Available Since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122046-plasmid-map-sequence-id-242459

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  18. 5VS7 (PP-BRD4)

    Plasmid
    #101264
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD4
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    101264-plasmid-map-sequence-id-193497

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  19. CHL1 T101A-eGFP

    Plasmid
    #117878
    Purpose
    protein expression
    Depositor
    Geoffrey Chang
    Insert
    CHL1 T101A-eGFP (AT5G40090 Mustard Weed)
    Expression
    Yeast
    Mutation
    T101A
    Promoter
    AOX
    Available Since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    117878-plasmid-map-sequence-id-230223

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  20. rk17s

    Plasmid
    #117437
    Purpose
    Expression of ARL14
    Depositor
    Richard Kahn
    Insert
    ARL14 (ARL14 Human)
    Expression
    Bacterial
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117437-plasmid-map-sequence-id-226358

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  21. MoClo adapted pUC57-Kan Level 2

    Plasmid
    #109218
    Purpose
    Non binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
    Depositor
    Elena Caro Bernat
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109218-plasmid-map-sequence-id-223588

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  25. pHH0103 TCERG1L WW domain #2

    Plasmid
    #104281
    Purpose
    Bacterial expression of WW domain #2 from TCERG1L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    TCERG1L WW domain #2 (TCERG1L Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104281-plasmid-map-sequence-id-202574

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  26. pHH0103 STXBP4 WW domain

    Plasmid
    #104265
    Purpose
    Bacterial expression of WW domain from STXBP4
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    STXBP4 WW domain (STXBP4 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104265-plasmid-map-sequence-id-203219

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  27. pHH0103 PIN1L WW domain

    Plasmid
    #104269
    Purpose
    Bacterial expression of WW domain from PIN1L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PIN1L WW domain (PIN1P1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104269-plasmid-map-sequence-id-203206

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  28. mr-GST N-term fusion

    Plasmid
    #112983
    Purpose
    For protein expression and purification of N-terminus of Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Expression
    Bacterial
    Mutation
    N-term only
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112983-plasmid-map-sequence-id-217464

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  29. pHH0103 SMURF1 WW domain #1

    Plasmid
    #104237
    Purpose
    Bacterial expression of WW domain #1 from SMURF1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF1 WW domain #1 (SMURF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104237-plasmid-map-sequence-id-204802

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  30. pHH0103 SMURF1 WW domain #2

    Plasmid
    #104238
    Purpose
    Bacterial expression of WW domain #2 from SMURF1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    SMURF1 WW domain #2 (SMURF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104238-plasmid-map-sequence-id-204801

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  31. pSC38

    Plasmid
    #104812
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.
    Depositor
    Nevin Young
    Insert
    Medtr3g107390
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    104812-plasmid-map-sequence-id-202765

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  32. DsRed-CPI-17-T38A-NES

    Plasmid
    #100791
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation cytosolic
    Depositor
    Joseph Gordon
    Insert
    PPP1R14A (PPP1R14A Human)
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100791-plasmid-map-sequence-id-193234

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  33. 5U9N (PP-BRD3)

    Plasmid
    #101255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101255-plasmid-map-sequence-id-197534

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  35. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

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  36. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

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  37. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  38. pDEST27-WFS1

    Plasmid
    #79276
    Purpose
    Mammalian expression of WFS1 with an N-terminal GST
    Depositor
    Rita Shiang
    Insert
    WFS1 (WFS1 Human)
    Tags
    GST
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pGEX-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid
    #75323
    Purpose
    GST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376
    Depositor
    Kalle Gehring
    Insert
    Sacsin Xenopus (Silurana) tropicalis 87-1376
    Tags
    GST-Tag
    Expression
    Bacterial
    Available Since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    75323-plasmid-map-sequence-id-145582

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  40. pGEX-SIRPT1-Ceratotherium simum simum

    Plasmid
    #75331
    Purpose
    GST fusion Ceratotherium simum simum Sacsin 89-1366
    Depositor
    Kalle Gehring
    Insert
    Sacsin Ceratotherium simum simum 89-1366
    Tags
    GST-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75331-plasmid-map-sequence-id-145569

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  41. pGEX-SIRPT1-Mustela putorius furo

    Plasmid
    #75333
    Purpose
    GST fusion Mustela putorius furo Sacsin 94-1381
    Depositor
    Kalle Gehring
    Insert
    Sacsin Mustela putorius furo 94-1381
    Tags
    GST-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75333-plasmid-map-sequence-id-145562

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  42. pColdII-SIRPT1-Ceratotherium-simum-simum

    Plasmid
    #78111
    Purpose
    Nt His-tag Ceratotherium-simum-simum Sacsin 89-1366
    Depositor
    Kalle Gehring
    Insert
    Sacsin Ceratotherium-simum-simum 89-1366
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78111-plasmid-map-sequence-id-145661

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  43. pColdII-SIRPT1-Mustela putorius furo

    Plasmid
    #78116
    Purpose
    Nt His-tag Mustela putorius furo Sacsin 94-1381
    Depositor
    Kalle Gehring
    Insert
    Sacsin Mustela putorius furo 94-1381
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78116-plasmid-map-sequence-id-145629

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  44. pREP81xV5_ccdB

    Plasmid
    #79019
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79019-plasmid-map-sequence-id-151964

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  45. pREP82xV5_ccdB

    Plasmid
    #79020
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79020-plasmid-map-sequence-id-147777

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  46. Mouse NCKX2-AS-short (pcDNA3.1+)

    Plasmid
    #75198
    Purpose
    Mammalian expression of SLC24A2
    Depositor
    Jonathan Lytton
    Insert
    SLC24A2 (Slc24a2 Mouse)
    Expression
    Mammalian
    Mutation
    alternatively spliced exon absent
    Promoter
    CMV
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75198-plasmid-map-sequence-id-142840

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  47. pJK241

    Plasmid
    #71693
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Tro404 to Phe
    Promoter
    T7 promoter
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71693-plasmid-map-sequence-id-129669

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  48. pJK699

    Plasmid
    #71691
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Asp371 to Asn
    Promoter
    T7 promoter
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71691-plasmid-map-sequence-id-129671

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  49. pJK708

    Plasmid
    #71692
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Trp400 to Phe
    Promoter
    T7 promoter
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71692-plasmid-map-sequence-id-129670

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  50. pJK195

    Plasmid
    #73321
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioC
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains silent mutation in Val428
    Promoter
    T7
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73321-plasmid-map-sequence-id-135029

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Showing: 9351 - 9400 of 167150 results