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pME-foxc1a

Plasmid

#132971

Purpose

full-length of foxc1a without stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

foxc1a (foxc1a Zebrafish)

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-mApg5(K130R)

Plasmid

#22957

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
p6589 MSCV-IP N-HAonly CHEK2

Plasmid

#34903

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHEK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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gh80

Plasmid

#106751

Purpose

expression of gRNA targeting MGAT4B

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT4B (MGAT4B Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-LC3A G120

Plasmid

#123091

Purpose

Expresses 3xFLAG-LC3A G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 121. Stop codon after G120.

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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MAP1LC3A-V1

Plasmid

#98569

Purpose

Expresses MAP1LC3A-V1

Depositor

Shahenda El-Naggar

Article

Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

Insert

MAP1LC3A (MAP1LC3A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3b(1454/3172)P2P-wt

Plasmid

#21404

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3A G120

Plasmid

#123071

Purpose

Gateway entry clone encoding human MAP1LC3A G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acid 121. Stop codon after G120.

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAZ_G-alpha

Plasmid

#109949

Purpose

Protein expression and purification of GNAZ_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAZ_G-alpha (GNAZ Human)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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3695 pGEX4T-3 MLL AD mut.E

Plasmid

#8867

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPADIMDFVL

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
gh6

Plasmid

#106690

Purpose

expression of gRNA targeting GALNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT6 (GALNT6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Gp88 in situ probe

Plasmid

#45628

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Gpr88 in situ probe (Gpr88 Mouse)

Use

In situ

Mutation

fragment contains bp# 2742-3255 of AB042408.1 or …

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
gh5

Plasmid

#106689

Purpose

expression of gRNA targeting GALNT5

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT5 (GALNT5 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS Jaws mCherry_tr

Plasmid

#111550

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mVenus_tr

Plasmid

#111552

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-SARA-V5-His6

Plasmid

#67452

Purpose

Mammalian expression of hSara with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only