We narrowed to 9,959 results for: Met

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Publication

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Tags

9xMyc

Expression

Yeast

Promoter

pADH1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-gst-xr

Plasmid

#214761

Purpose

Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

Depositor

Pimchai Chaiyen

Publication

Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

Insert

glutathione S-transferases fused with xylose reductase

Tags

Glutathione S-transferases

Expression

Bacterial

Promoter

T7

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Publication

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Expression

Mammalian

Mutation

E504K

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NES

Plasmid

#133022

Purpose

NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Publication

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NES

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-SARS-CoV-2 NSP8

Plasmid

#158359

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP8 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-eGFP-HF

Plasmid

#157735

Purpose

mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP-HF

Tags

His8-Flag

Expression

Mammalian

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12

Plasmid

#157709

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8

Plasmid

#157694

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-CBX1mut-PA-mCherry

Plasmid

#138251

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Publication

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Publication

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCoofy47

Plasmid

#55188

Purpose

Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Wnt9B STOP

Plasmid

#35882

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Use

Gateway Cloning

Mutation

SNP Met106-->Thr

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-RB (654-752)

Plasmid

#31496

Depositor

Julien Sage

Publication

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 654-752

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-ACP5

Plasmid

#31204

Depositor

Lynda Chin

Publication

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ACP5 (ACP5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
RCAS (A) Flu cPitx2 (CT#704)

Plasmid

#13928

Depositor

Cliff Tabin

Publication

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only