We narrowed to 18,451 results for: Met

1. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

   Plasmid

   #69221

   Purpose

   Expresses R1333K mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Lysine

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

   Plasmid

   #69222

   Purpose

   Expresses R1333S mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Serine

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMKO-sh_lnc-BATE1

   Plasmid

   #64866

   Purpose

   Expresses shRNA targeting lnc-BATE1

   Depositor

   Lei Sun

   Article

   Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

   Insert

   shRNA targeting lnc-BATE1
   

   Use

   Retroviral

   Promoter

   u6

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mIFP12-CAF1-C-10

   Plasmid

   #56246

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Tags

   mIFP12

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mIFP24-CAF1-C-10

   Plasmid

   #56266

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Tags

   mIFP24

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TALEN-CCR5L18-NtermN3

   Plasmid

   #51448

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) N3 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K150Q, K153Q, and R154Q

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALEN-CCR5R18-NtermN3

   Plasmid

   #51449

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) N3 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K150Q, K153Q, and R154Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFAB806

    Plasmid

    #47849

    Purpose

    BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    lambda tR2 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO4

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAB805

    Plasmid

    #47850

    Purpose

    BIOFAB reporter plasmid for measuring tonB termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    tonB terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO5

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFAB816

    Plasmid

    #47851

    Purpose

    BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    ilvGEDA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO6

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFAB807

    Plasmid

    #47852

    Purpose

    BIOFAB reporter plasmid for measuring RNAI termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    RNAI terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO7

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFAB820

    Plasmid

    #47855

    Purpose

    BIOFAB reporter plasmid for measuring tetAC termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    tetAC terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO10

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAB1910

    Plasmid

    #47859

    Purpose

    BIOFAB reporter plasmid for measuring rpoC termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rpoC terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO14

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFAB804

    Plasmid

    #47848

    Purpose

    BIOFAB reporter plasmid for measuring T3 early termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    T3 early terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO3

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAB3905

    Plasmid

    #47808

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 14 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-Map7-1783-end

    Plasmid

    #46071

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1783-end (Map7 Mouse)
    

    Expression

    Bacterial

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMT-Map7-856-1351

    Plasmid

    #46069

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-856-1351 (Map7 Mouse)
    

    Use

    Unspecified

    Expression

    Bacterial

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVf-EnhCB-lacZnls mir-122 1xBS

    Plasmid

    #35643

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    1 miR-122 target site
    

    Use

    AAV

    Promoter

    CB

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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