We narrowed to 4,442 results for: erf
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Plasmid#170734PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCDNA3_ReBphP-PCM_IRES_sfGFP
Plasmid#166917PurposeExpress ReBphP-PCM and sfGFPDepositorInsertsReBphP-PCM
sfGFP
ExpressionMammalianPromoterCMVAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT048A
Plasmid#159409PurposeConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23101-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromoterJ23101 and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT046A
Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialPromotertet and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29[dCas9]-1A(lacZ)
Plasmid#126979PurposeLevel 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationCas9 catalytically deadAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0808
Plasmid#91024PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0408
Plasmid#91010PurposeModule A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
Plasmid#138418PurposeCAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1DepositorInsertdCas9
UseLentiviralTagsBioTAP-tagPromoterEF1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianPromoterEFS-NSAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph
Plasmid#233067PurposePiggybac CRISPRi constructDepositorInsertKRAB-dCas9
UseCRISPRAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only