We narrowed to 10,102 results for: otos

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pAG1280 NLS-mCherry-FIREtag-NES

Plasmid

#205172

Purpose

expresses NLS-mCherry-FIREtag-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag-NES

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTP924 pHAGE2 CMV GFP

Plasmid

#199392

Purpose

Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

Plasmid

#66753

Purpose

miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Teto Ngn1

Plasmid

#62222

Purpose

Inducible expression of Ngn1

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pKin1A

Plasmid

#31607

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1A (Kif5a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Tags

myc tag

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAC-ZETAipi

Plasmid

#53284

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

pds

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJB158

Plasmid

#202601

Purpose

RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

Depositor

Athanasios Typas

Article

Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-EYFP

Plasmid

#53746

Purpose

contains fusion of human histone H2B to EYFP in pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EYFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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