We narrowed to 4,135 results for: pCMV mammalian

1. TdLanYFP-AURKA-Aquamarine

   Plasmid

   #228562

   Purpose

   Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Tags

   Aquamarine and TdLanYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AURKA-Aquamarine

   Plasmid

   #228564

   Purpose

   Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Tags

   Aquamarine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-hEAAT4

   Plasmid

   #32816

   Depositor

   Susan Amara

   Insert

   hEAAT4 (SLC1A6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

   Plasmid

   #223106

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

   Plasmid

   #223107

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

   Plasmid

   #223108

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

   Plasmid

   #223109

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AID-C12*-BE4max

   Plasmid

   #216729

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-BE4max (AICDA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-hEAAT3

   Plasmid

   #32815

   Depositor

   Susan Amara

   Insert

   hEAAT3 (SLC1A1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV EGFP-PPM1H-H153D

    Plasmid

    #200432

    Purpose

    Mammalian expression of H153D mutation in PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H153D

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-MAGE-112-429

    Plasmid

    #89128

    Purpose

    mammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    contains aa 112-429

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-Skp2

    Plasmid

    #89142

    Purpose

    mammalian expression of human Skp2 with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

    Insert

    Skp2 (SKP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CSII-PPM1H-mApple

    Plasmid

    #198473

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-mApple (PPM1H Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-ARF

    Plasmid

    #89123

    Purpose

    mammalian expression of p14-hARF with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CSII-PPM1H D288A-mApple

    Plasmid

    #198474

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-D288A-mApple (PPM1H Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. IKK-2 S177E S181E

    Plasmid

    #11105

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2090 HPV-11 E2

    Plasmid

    #10874

    Depositor

    Peter Howley

    Article

    Del Vecchio et al J Virol. 1992 Oct . 66(10):5949-58.

    Insert

    HPV11 E2
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

19. miCBE

    Plasmid

    #205413

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. HEpl016

    Plasmid

    #179991

    Purpose

    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    SiCas12i
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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