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AlkB-D135G

Plasmid

#160434

Purpose

A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

Depositor

Tao Pan

Article

Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

Insert

E. coli AlkB

Tags

His-tag

Expression

Bacterial

Mutation

Changed Aspartic acid 135 to Glycine; deleted the…

Promoter

T7

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A

Plasmid

#105694

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K31A

Plasmid

#105695

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A K31A

Plasmid

#105696

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 and 31 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-S98 (for p24)

Plasmid

#130717

Purpose

Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-S98

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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rs210-major

Plasmid

#113377

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 major allele

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs210-minor

Plasmid

#113378

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 minor allele

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-major

Plasmid

#113369

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 major allele

Use

Luciferase

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only
rs344-minor

Plasmid

#113366

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs34466261 minor allele

Use

Luciferase

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-minor

Plasmid

#113370

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 minor allele

Use

Luciferase

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-minor

Plasmid

#113374

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-major

Plasmid

#113373

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 major allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-minor

Plasmid

#113372

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-major

Plasmid

#113371

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 major allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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