We narrowed to 2,599 results for: Lox

1. pSicoR-Ef1a-mCh-Puro

   Plasmid

   #31845

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1821

   Plasmid

   #163092

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet^SEC (Lox511I)^::3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-DIO-GCaMP6s

   Plasmid

   #184284

   Purpose

   Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

   Depositor

   William Wisden

   Article

   Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

   Insert

   CCaMP6s
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synaptophysin

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGFAP-Cre

   Plasmid

   #40591

   Purpose

   Mouse astrocyte expression of Cre

   Depositor

   Albee Messing

   Article

   Zhuo et al Genesis. 2001 Oct;31(2):85-94.

   Insert

   Cre recombinase (GFAP Human, Mouse, Phage)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Mammalian

   Mutation

   Construct contains a nuclear-targeted Cre recombi…

   Promoter

   hGFAP promoter

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgNT/Cre

   Plasmid

   #66895

   Purpose

   Expresses a non-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

   Insert

   sgNT
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_070

   Plasmid

   #164265

   Purpose

   Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Fon-GCaMP6F

   Plasmid

   #137122

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV5 and AAV8

   Insert

   Con/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Fon-ChRmine-oScarlet

   Plasmid

   #137159

   Purpose

   Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-ChRmine-p2a-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   oScarlet E95D

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

   Plasmid

   #166970

   Purpose

   Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.

   Depositor

   Matthew Banghart

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG, T2A, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF-Con/Foff 2.0-iC++-EYFP

    Plasmid

    #137156

    Purpose

    Intersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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