We narrowed to 5,123 results for: puc

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pARA (pKD-G1)

Plasmid

#62680

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pARB (pKD-G2)

Plasmid

#62681

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATG (pKD-G5)

Plasmid

#62684

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATH (pKD-G6)

Plasmid

#62685

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP419/amiR-eGFP123

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATK (pKD-G9)

Plasmid

#62688

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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IVT-donor_opt(R2Tg)-GFP

Plasmid

#223246

Purpose

In vitro transcription of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

In vitro transcription

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEA038

Plasmid

#224547

Purpose

Blast-T2A-2xHA-FKPB12(dTagDegron) mouse Nipbl N-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

FKBP12F36V degron (dTAG system), blasticidin, 2xHA tag

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTetO-hOSK-CASH-1

Plasmid

#188977

Purpose

A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

Doxycycline inducible reprogramming donor cassette

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pL0-MtPT4Pro

Plasmid

#159414

Purpose

Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

MtPT4 Promoter

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-MtBCP1Pro

Plasmid

#159415

Purpose

Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

MtBCP1 Promoter

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b crRNA backbone

Plasmid

#103854

Purpose

For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has Service

Cloning Grade DNA

Insert

U6-BbsI-BbsI-PspCas13b DR-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO-EGFP

Plasmid

#237296

Purpose

Sleeping Beauty XPRESSO vector for eGFP expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHS2002

Plasmid

#239832

Purpose

Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB omegaRNA scaffold

Expression

Mammalian

Mutation

Replacement of A12-A57 with GAAA in omegaRNA scaf…

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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