We narrowed to 56,765 results for: plasmid
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Plasmid#187441PurposeLentiviral packaging plasmid expressing gag and pol.DepositorInsertsUseLentiviralAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDONRp5-p2 GFP-NShroom3-iLID
Plasmid#170976PurposeN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.DepositorInsertNShroom3 (Shroom3 Mouse)
UseGateway CloningTagseGFP and iLIDMutationFrom isoform 2, deleted amino acids 1389 - 1805,…Available SinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti F9-TetR-EGFP-IRES-PuroR
Plasmid#117049Purposelentiviral vector for expression of TetR-EGFP, for visualization of TetO repeatsDepositorInsertF9-TetR-EGFP
UseLentiviralTagsEGFPExpressionMammalianPromoterF9 promoterAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-SpCas9-HF1
Plasmid#108301PurposepX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCR5
Plasmid#98943PurposeMammalian expression plasmid for untagged human CCR5DepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralExpressionMammalianMutationMutated TET1 catalytic domainAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
BCL2L1_pLX307
Plasmid#98323PurposeLentiviral expression of BCL2L1DepositorAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BMP4_pLX307
Plasmid#98321PurposeLentiviral expression of BMP4DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)
Plasmid#90086PurposeThis plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianPromoterEFSAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTNNB1_WT
Plasmid#81734PurposeGateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTubb3
Plasmid#87116PurposeAAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITIDepositorInsertU6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
UseAAV and CRISPRAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorInsertHuman Brachyury knockin homology arms (TBXT Human)
Tags2A-EGFPAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL_U2AF1_S34F_mCherry
Plasmid#84019Purposeexpression of mutant U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianMutationS34FPromoterPGKAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…ExpressionBacterialPromoterhsp60 and hsp60(ribo)Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPb-NLG in FSW lentiviral vector
Plasmid#73148PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.DepositorInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
UseLentiviralTagsV5 tagExpressionMammalianPromoterSynapsinAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only