We narrowed to 30,456 results for: ica

1. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 35S::GeNL/pCAMBIA1301

   Plasmid

   #190070

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tom20-CIB-GFP

   Plasmid

   #117242

   Purpose

   Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   CIBN
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mito-Lapronic/pGW1

   Plasmid

   #140754

   Purpose

   FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

   Depositor

   Luis Felipe Barros

   Article

   Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

   Insert

   Mito-Lapronic
   

   Use

   Tags

   Mitochondrial Sequence Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. BRAC/pcDNA3

   Plasmid

   #51967

   Purpose

   mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

   Insert

   BRAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Cry2(1-531)-mCh-BAXS184E

    Plasmid

    #117238

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1 from Candida albicans

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LOTUS-V/pcDNA3

    Plasmid

    #87127

    Purpose

    Bioluminescent and ratiometric voltage indicator

    Depositor

    Takeharu Nagai

    Article

    Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

    Insert

    LOTUS-V
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tom20-CIB-stop

    Plasmid

    #117243

    Purpose

    Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    CIBN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT3-Neo

    Plasmid

    #69123

    Purpose

    Sleeping Beauty (SB) transposon with G418 resistance

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Insert

    Neo (codon optimized for human)
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET/LEGH

    Plasmid

    #108226

    Purpose

    Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

    Depositor

    Didier Picard

    Article

    Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

    Insert

    LBT-EGFP-His6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, lacO

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSopBC460S

    Plasmid

    #112514

    Purpose

    PsopB driven sopB (catalytically inactive) and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

    Insert

    sopB (catalytically inactive)/sigE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    SopB C460S

    Promoter

    PsopB

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a-TAX-L10-STEPtag

    Plasmid

    #159375

    Purpose

    Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    TAX-L10-STEPtag
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11a-STEPtag-L10-TAX

    Plasmid

    #159374

    Purpose

    Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    STEPtag-L10-TAX
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST96/LBT-MCS

    Plasmid

    #108227

    Purpose

    Bacterial expression expression of protein with N-terminal lanthanide binding tag

    Depositor

    Didier Picard

    Article

    Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

    Insert

    LBT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, lacO

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pADH118-17

    Plasmid

    #91727

    Purpose

    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217479

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1b
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Use

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET3a-FruK

    Plasmid

    #186256

    Purpose

    Overexpression of FruK for purification

    Depositor

    Liskin Swint-Kruse, Emile Van Schaftingen

    Article

    Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

    Insert

    1-phosphofructokinase (fruK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB_TRE3G_scFV-sfGFP-Ring1bCD

    Plasmid

    #235574

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ring1b (Rnf2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKRaION2-N1

    Plasmid

    #177802

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTXB1.Tat(48-57)-Cre recombinase

    Plasmid

    #165042

    Purpose

    Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Mxe Intein-Chitin binding domain and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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