We narrowed to 6,175 results for: nls

1. pSynapsin_NLS_GFP_P2A_Halo_GluA2

   Plasmid

   #170454

   Purpose

   Expresses halo tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV_BiSSTe10_dTomato_nlsdTomato

   Plasmid

   #213814

   Purpose

   AAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_dTomato_nlsdTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   dTomato_P2A_nls.dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BNLS

   Plasmid

   #50836

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP B
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

   Plasmid

   #176311

   Purpose

   Expresses NLS-pMag-CreC60 with tagBFP marker

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GANLS

   Plasmid

   #50852

   Purpose

   Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. RANLS

   Plasmid

   #50843

   Purpose

   Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

   Plasmid

   #176310

   Purpose

   Expresses CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSynapsin_NLS_GFP_P2A_His6_GluA2

   Plasmid

   #170453

   Purpose

   Expresses histidine tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Hexa-histidine

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45565

   Purpose

   Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   I-Sce I Nuclease
   BFP
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Use

    Tags

    nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45564

    Purpose

    Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    d14 eGFP donor
    I-Sce I Nuclease
    BFP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted n' 15 amino acids

    Promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBLO1811_arC9_noNLS_human

    Plasmid

    #74494

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    CRISPR

    Tags

    t2a mCherry (C-terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p413_Csy4NLS

    Plasmid

    #103139

    Purpose

    Expresses Csy4-NLS under TEF promoter

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    Csy4
    

    Use

    Tags

    NLS

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSP1594_2x_NLS

    Plasmid

    #110625

    Purpose

    Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    None
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSynapsin_NLS_GFP_P2A_SNAP_NR1

    Plasmid

    #170458

    Purpose

    Expresses SNAP tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge