We narrowed to 783 results for: p65
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Plasmid#12215DepositorInsertPak1 (PAK1 Human)
TagsGSTExpressionMammalianMutationAutoinhibitory domain (aa 83-149) with L107F muta…Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 P13A
Plasmid#12207DepositorInsertPak1 (PAK1 Human)
TagsMycExpressionMammalianMutationP13A: disrupts first polyproline region of Pak1Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
CFP-PBD(H83,86L)
Plasmid#11402DepositorInsertp21-activated kinase 1 (PAK1 Human)
TagsCFPExpressionMammalianMutationObtained with Histidine residues 83 and 86 change…Available SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSG-3'Hu-RelA
Plasmid#27276DepositorInsertRelA (RELA Human)
TagsGAL4 (aa1-147)ExpressionMammalianMutationContains only the transactivation domain (aa 292-…Available SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80 JuxtaK-142)
Plasmid#209013PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis-msfGFPExpressionBacterialMutationaa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80 JuxtaK-421)
Plasmid#209008PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutationsDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis-SUMOExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209016PurposeOverexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209011PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis and msfGFPExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1-PGM reporter
Plasmid#197278PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGMutationT15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT011-ZF6 1x FLAG-synTF-PPF
Plasmid#202501PurposeExpress "PPF" synTF (ZF6)DepositorInsertp65-ZF6
Tags1x FLAG and Palmitoylation-Palmitoylation-Farnesy…ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT009-ZF6 PM-synTF-1x FLAG
Plasmid#202499PurposeExpress "PM" synTF (ZF6)DepositorInsertp65-ZF6
Tags1x FLAG and Palmitoylation-Myristoylation SiteExpressionMammalianPromoterCMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) M173C
Plasmid#134696PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) F234C
Plasmid#134697PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) V304C
Plasmid#134698PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Syt1 C2AB(140) I367C
Plasmid#134699PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labelingDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B V304A,I367A IRES GFP
Plasmid#133821PurposeEncodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationV304A, I367APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A K189-192A IRES GFP
Plasmid#133823PurposeEncodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationK189-192APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A M173,F234A IRES GFP
Plasmid#133825PurposeEncodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationM173, F234APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only