We narrowed to 1,322 results for: pET28a

1. pET28a-DDX43-K292A

   Plasmid

   #134575

   Purpose

   Express human DDX43 mutant (K292A, ATPase dead) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-K292A (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (K292A)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-PKM2-G415R

   Plasmid

   #122693

   Purpose

   Express His-tagged PKM2 (G415R) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   G415R

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Beclin1 (1-242)

   Plasmid

   #24401

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 1-242 only.

   Promoter

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-GST-Q11

   Plasmid

   #185790

   Purpose

   Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   GST-Q11
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-BCA-Pep-H

   Plasmid

   #185782

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Pep-H
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-SpyTag002 DA-MBP

   Plasmid

   #102832

   Purpose

   Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002 DA-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Reactive aspartic acid has been mutated to an ala…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a His6Flag2AUF1(p42)

   Plasmid

   #135941

   Purpose

   Expresses AUF1p42 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a His6Flag2AUF1(p40)

    Plasmid

    #135940

    Purpose

    Expresses AUF1p40 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6his R.3-AR GUSpET28a+/pLysS

    Plasmid

    #61157

    Purpose

    N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

    Depositor

    Ichiro Matsumura, Andrew Ellington

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    GUSAR
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

    Promoter

    T7

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28A-p53 (1-73) (P27A)

    Plasmid

    #62068

    Purpose

    Expression of human p53 (1-73) (P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P27A

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-His6-UROD

    Plasmid

    #236739

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human (UROD Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-RANGAP1-CTD

    Plasmid

    #228947

    Purpose

    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

    Depositor

    Marcin Suskiewicz

    Insert

    RANGAP1-CTD (RANGAP1 Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGSSHHHHHHS…

    Expression

    Bacterial

    Mutation

    contains aa 418-587 only

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a:His-MBP-PKD

    Plasmid

    #237521

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4_pseudokinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pseudokinase region of RWT4

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a:His-MBP-RWT4Ca

    Plasmid

    #237518

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a:His-MBP-RWT4Ca_noKDup

    Plasmid

    #237535

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca_noKDup
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge