We narrowed to 54,949 results for: ran

1. pBactin-Akna-mOrange2

   Plasmid

   #196865

   Purpose

   Expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPOTv7-blast-lssmOrange

   Plasmid

   #179795

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean, Keith Gull

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   lss mOrange
   

   Use

   Unspecified

   Promoter

   read through

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. membrane-mCherry-SpVasa-C3

   Plasmid

   #185587

   Purpose

   to recruit Vasa-C3 to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa-C3
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry and membrane targeting sequence from Neur…

   Mutation

   C3 mutant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. membrane-mCherry-SpVasa

   Plasmid

   #185586

   Purpose

   to recruit Vasa to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry, S-Tag and membrane targeting sequence fr…

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. promoter-less mRFP1_Orange

   Plasmid

   #183143

   Purpose

   BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Orange

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   RBS, mRFP1E_Orange
   

   Use

   Synthetic Biology

   Mutation

   BioBrick sites removed

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. membrane-HsPLK1-dead

   Plasmid

   #185572

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   PLK1-dead (PLK1 Human)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   membrane targeting sequence from Neuromodulin (ML…

   Mutation

   K82R kinase-dead mutant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE_Tubb3 GFP(Y39TAG) KI

   Plasmid

   #182678

   Purpose

   Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and chicken beta-actin promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE_NFL linker-3xFLAG KI

   Plasmid

   #182679

   Purpose

   Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and chicken beta-actin promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE Tubb3-2xGFP KI

   Plasmid

   #183443

   Purpose

   GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and 2xGFP donor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE Tubb3-3xGFP KI

    Plasmid

    #183444

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 3xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)_Granulin 7-no linker

    Plasmid

    #176931

    Purpose

    express Granulin 7 without linker 8 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 7 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)_Granulin 7+linker8

    Plasmid

    #176930

    Purpose

    express Granulin 7 with linker 8 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 7+linker8 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)_Granulin 6-no linker

    Plasmid

    #176929

    Purpose

    express Granulin 6 without linker 7 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 6 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)_Granulin 6+linker7

    Plasmid

    #176928

    Purpose

    express Granulin 6 with linker 7 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 6+linker7 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)_Granulin 5-no linker

    Plasmid

    #176927

    Purpose

    express Granulin 5 without linker 6 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 5 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)_Granulin 5+linker6

    Plasmid

    #176926

    Purpose

    express Granulin 5 with linker 6 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 5+linker6 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)_Granulin 3-no linker

    Plasmid

    #176923

    Purpose

    express Granulin 3 without linker 4 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 3 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)_Granulin 3+linker4

    Plasmid

    #176922

    Purpose

    express Granulin 3 with linker 4 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 3+linker4 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)_Granulin 1-no linker

    Plasmid

    #176919

    Purpose

    express Granulin 1 without linker 2 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 1 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP-EGFP-RanGAP*

    Plasmid

    #175238

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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