We narrowed to 6,467 results for: poly

1. pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)

   Plasmid

   #233871

   Purpose

   Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   El Vadh (Elen_0497 Eggerthella lenta)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

   Plasmid

   #233870

   Purpose

   Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (–)-Eldh1 (Elen_0511 Eggerthella lenta)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

   Plasmid

   #233869

   Purpose

   Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (+)-Eddh (Elen_0501 Eggerthella lenta)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCARGO-RMCE-Xist

   Plasmid

   #233268

   Purpose

   CARGO plasmid containing ~22kb dox-inducible Xist transgene

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Insert

   22kb Xist gene, including introns
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)

   Plasmid

   #225305

   Purpose

   Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (+)-Eldh1, (+)-Eldh2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

   Plasmid

   #233875

   Purpose

   Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZH81 pET21b Rat1-His

   Plasmid

   #231650

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   Rat1 (RAT1 Budding Yeast)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EYFP-Parkin

   Plasmid

   #210292

   Purpose

   mitophagy marker

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   EYFP-Parkin
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mActA-FKBP-mCherry

   Plasmid

   #210295

   Purpose

   ActuAtor for FKBP-FRB CID

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   mActA-FKBP-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ECFP-FRB-GiantinS

    Plasmid

    #210296

    Purpose

    Golgi marker and anchor for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-GiantinS
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EYFP-FKBP-DN-N-WASP

    Plasmid

    #210301

    Purpose

    control for targeted actin nucleation

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    EYFP-FKBP-DN-N-WASP
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EYFP-FKBP-N-WASP

    Plasmid

    #210302

    Purpose

    targeted actin nucleation

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    EYFP-FKBP-N-WASP
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hSyn-GEM

    Plasmid

    #187648

    Purpose

    Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    PfV
    

    Use

    AAV

    Tags

    HaloTag and SNAP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a- mCherry-FIVAR-HIS-ybbR

    Plasmid

    #169138

    Purpose

    E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    mCherry
    

    Use

    Tags

    6x Histag, FIVAR tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cut190*_pET21b

    Plasmid

    #215404

    Purpose

    Expression construct for Cut190* gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Cut190*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S226P/R228S

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RoL10X_pET29b

    Plasmid

    #215427

    Purpose

    Expression construct for RoL10X gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    RoL10X
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NylB-SCY_pET29b

    Plasmid

    #215415

    Purpose

    Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac1-His6-E3(1)-CUbo-TwinStrep

    Plasmid

    #212712

    Purpose

    Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    6xHis and CUbo-TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHyg-CUbo(K48R/K63R)-His6

    Plasmid

    #212780

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge