We narrowed to 18,548 results for: Mut

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11015

Plasmid

#117499

Purpose

FAST-Red selectable marker Golden Gate Level 1 Position 1

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI

Tags

RFP

Expression

Plant

Promoter

AtOLE1

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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DJ-1 pET15b

Plasmid

#60678

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
BCJJ125

Plasmid

#117515

Purpose

Cas9_3 (with intron) Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_3(intron):GCTT:BsaI

Use

CRISPR

Tags

2xFLAG and NLS

Expression

Plant

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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FLT3-MSCV-IRES-GFP

Plasmid

#184778

Purpose

Expresses wildtype FLT3 in mammalian cells

Depositor

Timothy Ley

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD1iresGFP

Plasmid

#45025

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD1 (Neurod1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN4 WT

Plasmid

#59320

Purpose

Expression plasmid of HA-tagged wildtype human Neuroligin 4

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN4 (NLGN4X Human)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCBL101

Plasmid

#199722

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

NPTII

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLI*-HA

Plasmid

#211461

Purpose

Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI mutant

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCBL102-RUBY

Plasmid

#210379

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Expression

Plant

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pUCSh5vio-2300

Plasmid

#170987

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300 violacein

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

placI

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pX1

Plasmid

#46848

Purpose

Vector modified from pBluescript SK(+) by adding more cloning sites

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Article

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Expression

Mammalian

Available Since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B CPM2

Plasmid

#121163

Purpose

Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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wt HIV-1 integrase in pET15b

Plasmid

#61668

Purpose

wild type HIV-1 integrase in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

wild type HIV-1 integrase

Tags

His

Promoter

T7 promotor

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only